Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hat Yai, 90112, Thailand.
Orthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, 90112, Songkhla, Thailand; Guiyang Hospital of Stomatology, Guiyang, 550002, People's Republic of China.
J Mech Behav Biomed Mater. 2024 May;153:106500. doi: 10.1016/j.jmbbm.2024.106500. Epub 2024 Mar 8.
One strategy to correct alveolar bone defects is use of bioactive bone substitutes to maintain the structure of defect site and facilitate cells and vessels' ingrowth. This study aimed to fabricate and characterize the freeze-dried bone regeneration scaffolds composed of polymeric Type I collagen, nano Beta-tricalcium phosphate (β-TCP), and gelatin. The stable structures of scaffolds were obtained by thermal crosslinking and EDC/NHS ((1-ethyl-3-(3-dimethylaminopropyl) carbodiimide)/(N-hydroxysuccinimide)) chemical crosslinking processes. Subsequently, the physicochemical and biological properties of the scaffolds were characterized and assessed. The results indicated the bioactive composite scaffolds containing 10% and 20% (w/v) nano β-TCP exhibited suitable porosity (84.45 ± 25.43 nm, and 94.51 ± 14.69 nm respectively), a rapid swelling property (reaching the maximum swelling rate at 1 h), excellent degradation resistance (residual mass percentage of scaffolds higher than 80% on day 90 in PBS and Type I collagenase solution respectively), and sustained calcium release capabilities. Moreover, they displayed outstanding biological properties, including superior cell viability, cell adhesion, and cell proliferation. Additionally, the scaffolds containing 10% and 20% (w/v) nano β-TCP could promote the osteogenic differentiation of MC3T3-E1. Therefore, the bioactive composite scaffolds containing 10% and 20% (w/v) nano β-TCP could be further studied for being used to treat alveolar bone defects in vivo.
一种纠正牙槽骨缺损的策略是使用生物活性骨替代物来维持缺损部位的结构，并促进细胞和血管的长入。本研究旨在制备和表征由聚合型 I 型胶原、纳米β-磷酸三钙（β-TCP）和明胶组成的冻干骨再生支架。支架的稳定结构通过热交联和 EDC/NHS（1-乙基-3-（3-二甲基氨基丙基）碳二亚胺/（N-羟基琥珀酰亚胺））化学交联过程获得。随后，对支架的物理化学和生物学性质进行了表征和评估。结果表明，含有 10%和 20%（w/v）纳米β-TCP 的生物活性复合支架具有适宜的孔隙率（分别为 84.45±25.43nm 和 94.51±14.69nm）、快速溶胀性能（在 1h 内达到最大溶胀率）、优异的抗降解能力（在 PBS 和 I 型胶原酶溶液中第 90 天支架的残留质量百分比均高于 80%）和持续的钙释放能力。此外，它们还表现出优异的生物学特性，包括较高的细胞活力、细胞黏附和细胞增殖。此外，含有 10%和 20%（w/v）纳米β-TCP 的支架能够促进 MC3T3-E1 的成骨分化。因此，含有 10%和 20%（w/v）纳米β-TCP 的生物活性复合支架可进一步研究用于治疗体内牙槽骨缺损。
