College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, Jiangsu, China; Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, Jiangsu, China.
Sci Total Environ. 2024 May 10;924:171639. doi: 10.1016/j.scitotenv.2024.171639. Epub 2024 Mar 12.
The utilization of solar energy and fast-growing heterotrophic microbes for biofuel production has been recognized as a promising approach to achieve carbon neutrality and address energy crisis. In this work, we synthesized different kinds of photocatalysts based on graphitic carbon nitride (g-CN). We found that carbon dots modified-graphitic carbon nitride (CDs-g-CN) showed the highest photocatalytic activity. Subsequently, we developed a photocatalyst-microbe hybrid (PMH) system by combining CDs-g-CN with an oleaginous yeast strain, Cutaneotrichosporon dermatis ZZ-46. Under visible light irradiation, the lipid yield of this PMH system reached 1.70 g/L at 120 h, representing a 36 % increase compared to the control. The photocatalytic reaction-induced ROS and the reductive photogenerated electrons facilitated ZZ-46 cells to synthesize more lipids. Furthermore, the fermentation residual of this PMH system was reutilized to prepare biochar via pyrolysis. The biochar generated at 550 °C (BC-550) demonstrated exceptional adsorption capabilities, particularly with a 57 % adsorption rate for methylene blue (MB), and maintained its perfect adsorption efficacy even after five regeneration cycles. These results offer promising avenues for addressing energy shortages and environmental contamination.
利用太阳能和快速生长的异养微生物生产生物燃料已被认为是实现碳中和和应对能源危机的一种很有前途的方法。在这项工作中，我们合成了基于石墨相氮化碳（g-CN）的不同类型的光催化剂。我们发现，碳点修饰的石墨相氮化碳（CDs-g-CN）表现出最高的光催化活性。随后，我们通过将 CDs-g-CN 与一种产油酵母菌株 Cutaneotrichosporon dermatis ZZ-46 结合，开发了一种光催化剂-微生物混合（PMH）系统。在可见光照射下，该 PMH 系统的脂质产率在 120 小时达到 1.70 g/L，与对照组相比增长了 36%。光催化反应诱导的活性氧（ROS）和还原性光生电子有助于 ZZ-46 细胞合成更多的脂质。此外，该 PMH 系统的发酵残余物可通过热解再利用来制备生物炭。在 550°C 下生成的生物炭（BC-550）表现出优异的吸附能力，对亚甲基蓝（MB）的吸附率达到 57%，即使经过五次再生循环，其吸附效果仍保持完美。这些结果为解决能源短缺和环境污染问题提供了有前景的途径。
