Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Pelotas, RS, Brazil.
Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Neuroquímica, Inflamação e Câncer, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Pelotas, RS, Brazil.
Neurosci Lett. 2024 Mar 15;826:137730. doi: 10.1016/j.neulet.2024.137730. Epub 2024 Mar 12.
Considering that the combination of dasatinib and quercetin (D + Q) demonstrated a neuroprotective action, as well as that females experience a decline in hormonal levels during aging and this is linked to increased susceptibility to Alzheimer's disease, in this study we evaluated the effect of D + Q on inflammatory and oxidative stress markers and on acetylcholinesterase and Na, K-ATPase activities in brain of female mice.
Female C57BL/6 mice were divided in Control and D (5 mg/kg) + Q (50 mg/kg) treated. Treatment was administered via gavage for three consecutive days every two weeks starting at 30 days of age. The animals were euthanized at 6 months of age and at 14 months of age.
Results indicate an increase in reactive species (RS), thiol content and lipid peroxidation followed by a reduction in nitrite levels and superoxide dismutase, catalase and glutathione S-transferase activity in the brain of control animals with age. D+Q protected against age-associated increase in RS and catalase activity reduction. Acetylcholinesterase activity was increased, while Na, K-ATPase activity was reduced at 14 months of age and D+Q prevented this reduction.
These data demonstrate that D+Q can protect against age-associated neurochemical alterations in the female brain.
鉴于达沙替尼和槲皮素（D+Q）联合具有神经保护作用，并且女性在衰老过程中经历激素水平下降，这与阿尔茨海默病的易感性增加有关，因此在本研究中，我们评估了 D+Q 对雌性小鼠大脑中炎症和氧化应激标志物以及乙酰胆碱酯酶和 Na、K-ATP 酶活性的影响。
将 C57BL/6 雌性小鼠分为对照组和 D（5mg/kg）+Q（50mg/kg）治疗组。治疗通过灌胃给药，每两周连续给药 3 天，从 30 天龄开始。动物在 6 个月和 14 个月大时被安乐死。
结果表明，随着年龄的增长，对照组动物大脑中的活性物质（RS）、巯基含量和脂质过氧化增加，随后亚硝酸盐水平和超氧化物歧化酶、过氧化氢酶和谷胱甘肽 S-转移酶活性降低。D+Q 可防止与年龄相关的 RS 增加和过氧化氢酶活性降低。乙酰胆碱酯酶活性增加，而 Na、K-ATP 酶活性在 14 个月时降低，D+Q 可防止这种降低。
这些数据表明，D+Q 可以防止雌性大脑中与年龄相关的神经化学改变。
