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Sox9 在非梗阻性无精子症中通过 UTF1 的下调和 POU5F1 的介导作用。

Sox9 downregulation in non-obstructive azoospermia by UTF1 and mediator role of POU5F1.

机构信息

Faculty of Biotechnology, Amol University of Special Modern Technologies, 49767, Amol, P.O. Box: 49767, Iran.

Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

BMC Res Notes. 2024 Mar 14;17(1):77. doi: 10.1186/s13104-024-06711-0.

DOI:10.1186/s13104-024-06711-0
PMID:38486279
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10941352/
Abstract

BACKGROUND

Spermatogenesis is the process of producing mature sperm from Spermatogonial stem cells (SSCs) and this process requires a complex cooperation of different types of somatic and germ cells. Undifferentiated spermatogonia initiate the spermatogenesis and Sertoli cells as the only somatic cells inside of the seminiferous tubule play a key role in providing chemical and physical requirements for normal spermatogenesis, here, we investigated the dysfunction of these cells in non-obstructive azoospermia.

MATERIAL AND METHOD

In this study, we analyzed the expression of sox9 and UTF1 in the non-obstructive human testis by immunohistochemistry. Moreover, we used the KEGG pathway and bioinformatics analysis to reveal the connection between our object genes and protein.

RESULTS

The immunohistochemistry analysis of the non-obstructive human seminiferous tubule showed low expression of Sox9 and UTF1 that was detected out of the main location of the responsible cells for these expressions. Our bioinformatics analysis clearly and strongly indicated the relation between UTF1 in undifferentiated spermatogonia and Sox9 in Sertoli cells mediated by POU5F1.

CONCLUSION

Generally, this study showed the negative effect of POU5F1 as a mediator between Sertoli cells as the somatic cells within seminiferous tubules and undifferentiated spermatogonia as the spermatogenesis initiator germ cells in non-obstructive conditions.

摘要

背景

精子发生是从精原干细胞（SSC）产生成熟精子的过程，这个过程需要不同类型的体细胞和生殖细胞的复杂合作。未分化的精原细胞启动精子发生，而作为生精小管内唯一体细胞的支持细胞在提供正常精子发生的化学和物理需求方面起着关键作用，在这里，我们研究了这些细胞在非阻塞性无精子症中的功能障碍。

材料和方法

在这项研究中，我们通过免疫组织化学分析了非阻塞性人类睾丸中 sox9 和 UTF1 的表达。此外，我们使用了 KEGG 通路和生物信息学分析来揭示我们的目标基因和蛋白质之间的联系。

结果

非阻塞性人类精曲小管的免疫组织化学分析显示 Sox9 和 UTF1 的表达水平较低，这些表达的主要位置在负责这些表达的细胞之外。我们的生物信息学分析清楚而强烈地表明，POU5F1 介导了未分化精原细胞中的 UTF1 和支持细胞中的 Sox9 之间的关系。

结论

总的来说，这项研究表明，在非阻塞性条件下，作为生精小管内体细胞的支持细胞和作为精子发生起始生殖细胞的未分化精原细胞之间的 POU5F1 作为介质具有负效应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd91/10941352/fd6ca9c63323/13104_2024_6711_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd91/10941352/9e7f2ef361d8/13104_2024_6711_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd91/10941352/17fbba63a894/13104_2024_6711_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd91/10941352/fd6ca9c63323/13104_2024_6711_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd91/10941352/9e7f2ef361d8/13104_2024_6711_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd91/10941352/17fbba63a894/13104_2024_6711_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd91/10941352/fd6ca9c63323/13104_2024_6711_Fig4_HTML.jpg

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