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探索 GhN/AINV23 的作用：对植物生长、发育和耐旱性的影响。

Exploring the role of GhN/AINV23: implications for plant growth, development, and drought tolerance.

机构信息

National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, 572024, Sanya, Hainan, China.

National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences (CAAS), 455000, Anyang, Henan, China.

出版信息

Biol Direct. 2024 Mar 14;19(1):22. doi: 10.1186/s13062-024-00465-2.

DOI:10.1186/s13062-024-00465-2
PMID:38486336
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10938729/
Abstract

BACKGROUND

Neutral/alkaline invertases (N/AINVs) play a crucial role in plant growth, development, and stress response, by irreversibly hydrolyzing sucrose into glucose and fructose. However, research on cotton in this area is limited. This study aims to investigate GhN/AINV23, a neutral/alkaline invertase in cotton, including its characteristics and biological functions.

RESULTS

In our study, we analyzed the sequence information, three-dimensional (3D) model, phylogenetic tree, and cis-elements of GhN/AINV23. The localization of GhN/AINV23 was determined to be in the cytoplasm and cell membrane. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that GhN/AINV23 expression was induced by abscisic acid (ABA), exogenous sucrose and low exogenous glucose, and inhibited by high exogenous glucose. In Arabidopsis, overexpression of GhN/AINV23 promoted vegetative phase change, root development, and drought tolerance. Additionally, the virus-induced gene silencing (VIGS) assay indicated that the inhibition of GhN/AINV23 expression made cotton more susceptible to drought stress, suggesting that GhN/AINV23 positively regulates plant drought tolerance.

CONCLUSION

Our research indicates that GhN/AINV23 plays a significant role in plant vegetative phase change, root development, and drought response. These findings provide a valuable foundation for utilizing GhN/AINV23 to improve cotton yield.

摘要

背景

中性/碱性转化酶（N/AINV）通过不可逆地将蔗糖水解为葡萄糖和果糖，在植物生长、发育和应激反应中起着至关重要的作用。然而，该领域对棉花的研究有限。本研究旨在研究棉花中的 GhN/AINV23，一种中性/碱性转化酶，包括其特征和生物学功能。

结果

在我们的研究中，我们分析了 GhN/AINV23 的序列信息、三维（3D）模型、系统发育树和顺式元件。GhN/AINV23 的定位被确定为在细胞质和细胞膜中。定量实时聚合酶链反应（qRT-PCR）结果表明，GhN/AINV23 的表达受脱落酸（ABA）、外源蔗糖和低外源葡萄糖诱导，受高外源葡萄糖抑制。在拟南芥中，过表达 GhN/AINV23 促进营养生长阶段转变、根系发育和耐旱性。此外，病毒诱导的基因沉默（VIGS）试验表明，抑制 GhN/AINV23 的表达使棉花更容易受到干旱胁迫的影响，这表明 GhN/AINV23 正向调节植物的耐旱性。

结论

我们的研究表明，GhN/AINV23 在植物营养生长阶段转变、根系发育和干旱响应中起着重要作用。这些发现为利用 GhN/AINV23 提高棉花产量提供了有价值的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/cb46d47e1312/13062_2024_465_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/1c5072ecb95a/13062_2024_465_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/7cf8bd1b4b39/13062_2024_465_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/e5cf48c90411/13062_2024_465_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/009c9ba7374e/13062_2024_465_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/cb46d47e1312/13062_2024_465_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/1c5072ecb95a/13062_2024_465_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/7cf8bd1b4b39/13062_2024_465_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/e5cf48c90411/13062_2024_465_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/009c9ba7374e/13062_2024_465_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71e/10938729/cb46d47e1312/13062_2024_465_Fig5_HTML.jpg

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