Shahabi Saeed, Azizi Kourosh, Asgari Qasem, Sarkari Bahador
Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Research Center for Health Sciences, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Can J Infect Dis Med Microbiol. 2024 Mar 7;2024:4896873. doi: 10.1155/2024/4896873. eCollection 2024.
Cutaneous leishmaniasis is of particular importance in southern Iran. This study aimed to investigate the infection of rodents with in an urban area of Fars Province, located in southern Iran. Rodents were trapped and samples from the liver, spleen, and skin were collected. Impression smears were prepared from these tissues and any skin lesions and were examined microscopically. In addition, a portion of the samples were preserved for subsequent DNA extraction. A total of 41 rodents belonging to three species were caught from 10 trapping stations in gardens or houses within the area. The caught rodent species were ( = 25, 60.97%), ( = 15, 36.58%), and ( = 1, 2.5%). amastigotes were seen in the spleen tissue smear of 6 (2.43%) of the rodents, including 4 of and 2 of . Skin lesions were observed on the muzzles of two and one . Samples taken from these lesions tested positive for infection. DNA was detected in 18 (43.9%) rodents, including 11 6 , and one , based on DNA sequencing of the ITS2 gene and PCR of the kDNA. Phylogenetic reconstruction revealed that the parasite infecting the rodents was . The detection of infection in these rodents in urban areas raises concerns about the urbanization of cutaneous leishmaniasis caused by . This urbanization poses unique challenges for control and prevention efforts.
皮肤利什曼病在伊朗南部尤为重要。本研究旨在调查伊朗南部法尔斯省一个城市地区啮齿动物感染[寄生虫名称未给出]的情况。捕获啮齿动物并采集肝脏、脾脏和皮肤样本。从这些组织以及任何皮肤病变处制备印片，进行显微镜检查。此外，保存一部分样本用于后续的DNA提取。在该地区的花园或房屋内的10个诱捕站共捕获了属于三个物种的41只啮齿动物。捕获的啮齿动物物种为[物种名称未给出]（n = 25，60.97%）、[物种名称未给出]（n = 15，36.58%）和[物种名称未给出]（n = 1，2.5%）。在6只（2.43%）啮齿动物的脾脏组织涂片中发现了[寄生虫名称未给出]无鞭毛体，其中包括4只[物种名称未给出]和2只[物种名称未给出]。在两只[物种名称未给出]和一只[物种名称未给出]的口鼻部观察到皮肤病变。从这些病变处采集的样本检测出[寄生虫名称未给出]感染呈阳性。基于ITS2基因的DNA测序和kDNA的PCR，在18只（43.9%）啮齿动物中检测到[寄生虫名称未给出]DNA，包括11只[物种名称未给出]、6只[物种名称未给出]和1只[物种名称未给出]。系统发育重建显示感染啮齿动物的寄生虫是[寄生虫名称未给出]。在这些城市地区的啮齿动物中检测到[寄生虫名称未给出]感染引发了对由[寄生虫名称未给出]引起的皮肤利什曼病城市化的担忧。这种城市化给控制和预防工作带来了独特的挑战。
