State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, P. R. China.
Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, P. R. China.
Environ Sci Technol. 2024 Apr 9;58(14):6284-6295. doi: 10.1021/acs.est.3c10030. Epub 2024 Mar 15.
The anammox dynamic membrane bioreactor (DMBR) is promising in applications with enhanced anammox biomass enrichment and fouling alleviation. However, the metabolic mechanism underlying the functional features of anammox sludge and the biofilm membrane is still obscure. We investigated the metabolic networks of anammox sludge and membrane biofilm in the DMBR. The cooperation between anammox and dissimilatory nitrate reduction to ammonium processes favored the robust anammox process in the DMBR. The rapid bacterial growth occurred in the DMBR sludge with 1.33 times higher biomass yield compared to the MBR sludge, linked to the higher activities of lipid metabolism, nucleotide metabolism, and B vitamin-related metabolism of the DMBR sludge. The metabolism of the DMBR biofilm microbial community benefited the fouling alleviation that the abundant fermentative bacteria and their cooperation with the anammox sludge microbial community promoted organics degradation. The intensified degradation of foulants by the DMBR biofilm community was further evidenced by the active carbohydrate metabolism and the upregulated vitamin B intermediates in the biofilms of the DMBR. Our findings provide insights into key metabolic mechanisms for enhanced biomass enrichment and fouling control of the anammox DMBR, guiding manipulations and applications for overcoming anammox biomass loss in the treatment of wastewater under detrimental environmental conditions.
厌氧氨氧化动态膜生物反应器（DMBR）在强化厌氧氨氧化生物量富集和减轻污染方面具有应用前景。然而，厌氧氨氧化污泥和生物膜功能特征的代谢机制仍不清楚。我们研究了 DMBR 中厌氧氨氧化污泥和膜生物膜的代谢网络。厌氧氨氧化和异化硝酸盐还原为氨过程的协同作用有利于 DMBR 中稳健的厌氧氨氧化过程。DMBR 污泥中的细菌生长迅速，生物量产率比 MBR 污泥高 1.33 倍，这与 DMBR 污泥中脂质代谢、核苷酸代谢和 B 族维生素相关代谢的更高活性有关。DMBR 生物膜微生物群落的代谢有利于减轻污染，丰富的发酵细菌及其与厌氧氨氧化污泥微生物群落的合作促进了有机物的降解。DMBR 生物膜群落对污染物的强化降解进一步得到了证实，即 DMBR 生物膜中碳水化合物代谢活跃，维生素 B 中间产物上调。我们的研究结果为强化厌氧氨氧化 DMBR 中生物量富集和控制污染的关键代谢机制提供了新的见解，指导了在恶劣环境条件下处理废水时克服厌氧氨氧化生物量损失的操作和应用。
