δ-阿片受体在调节心肌细胞缺氧/复氧损伤耐受性中的作用。
The Role of δ-Opioid Receptors in the Regulation of Tolerance of Isolated Cardiomyocytes to Hypoxia and Reoxygenation.
机构信息
Cardiology Research Institute, Tomsk National Research Medical Center, Tomsk, Russia.
Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino, Moscow region, Russia.
出版信息
Bull Exp Biol Med. 2024 Feb;176(4):433-436. doi: 10.1007/s10517-024-06041-y. Epub 2024 Mar 15.
Hypoxia (20 min) and reoxygenation (30 min) were simulated on isolated rat cardiomyocytes to evaluate the cytoprotective effect of selective δ-opioid receptor agonist deltorphin II, opioid receptor antagonist naloxone methiodide, μ-opioid receptor antagonist CTAP, κ-opioid receptor antagonist nor-binaltorphimine, ε-opioid receptor antagonist BNTX, and δ-opioid receptors naltriben. Deltorphin II was administered 5 min before reoxygenation, antagonists were administered 10 min before reoxygenation. The cytoprotective effect of deltorphin II was assessed by the number of cardiomyocytes survived after hypoxia/reoxygenation, as well as by the lactate dehydrogenase content in the incubation medium. It has been established that the cytoprotective effect of deltorphin II occurs at a concentration of 64 nmol/liter and is associated with activation of δ-opioid receptors.
缺氧（20 分钟）和复氧（30 分钟）在分离的大鼠心肌细胞上进行模拟，以评估选择性 δ 阿片受体激动剂 δ 啡肽 II、阿片受体拮抗剂纳洛酮甲碘化物、μ 阿片受体拮抗剂 CTAP、κ 阿片受体拮抗剂诺宾那替林、ε 阿片受体拮抗剂 BNTX 和 δ 阿片受体纳曲酮的细胞保护作用。δ 啡肽 II 在复氧前 5 分钟给予，拮抗剂在复氧前 10 分钟给予。通过缺氧/复氧后存活的心肌细胞数量以及孵育培养基中乳酸脱氢酶的含量来评估 δ 啡肽 II 的细胞保护作用。已经证实，δ 啡肽 II 的细胞保护作用发生在 64nmol/L 的浓度下，与 δ 阿片受体的激活有关。
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