Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Joint International Research Laboratory of Metabolic and Developmental Sciences, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Cell Rep. 2024 Mar 26;43(3):113950. doi: 10.1016/j.celrep.2024.113950. Epub 2024 Mar 13.
Despite extensive research, the origin and evolution of the chloroplast division machinery remain unclear. Here, we employ recently sequenced genomes and transcriptomes of Archaeplastida clades to identify the core components of chloroplast division and reconstruct their evolutionary histories, respectively. Our findings show that complete division ring structures emerged in Charophytes. We find that Glaucophytes experienced strong selection pressure, generating diverse variants adapted to the changing terrestrial environments. By integrating the functions of chloroplast division genes (CDGs) annotated in a workflow developed using large-scale multi-omics data, we further show that dispersed duplications acquire more species-specific functions under stronger selection pressures. Notably, PARC6, a dispersed duplicate CDG, regulates leaf color and plant growth in Solanum lycopersicum, demonstrating neofunctionalization. Our findings provide an integrated perspective on the functional evolution of chloroplast division machinery and highlight the potential of dispersed duplicate genes as the primary source of adaptive evolution of chloroplast division.
尽管进行了广泛的研究，但叶绿体分裂机制的起源和进化仍不清楚。在这里，我们利用最近测序的藻类门各演化支的基因组和转录组，分别鉴定叶绿体分裂的核心组件并重建它们的进化历史。我们的研究结果表明，完整的分裂环结构出现在轮藻中。我们发现，蓝藻经历了强烈的选择压力，产生了多种适应陆地环境变化的变体。通过整合在使用大规模多组学数据开发的工作流程中注释的叶绿体分裂基因 (CDG) 的功能，我们进一步表明，分散重复在更强的选择压力下获得更多物种特异性的功能。值得注意的是，PARC6，一个分散的重复 CDG，调节番茄中的叶片颜色和植物生长，表明新功能化。我们的研究结果提供了一个综合的视角，了解叶绿体分裂机制的功能进化，并强调分散重复基因作为叶绿体分裂适应性进化的主要来源的潜力。
