Institute of Metabolic Science, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 0QQ, UK; Cambridge Universities NHS Foundation Trust, Cambridge CB2 0QQ UK.
Institute of Metabolic Science, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 0QQ, UK; Cambridge Universities NHS Foundation Trust, Cambridge CB2 0QQ UK; Current addresses: Leicester Diabetes Centre, University of Leicester, Gwendoline Road, Leicester LE5 4PW, UK; and University Hospitals Leicester, Leicester General Hospitals, Gwendoline Road, Leicester LE5 4PW, UK.
Peptides. 2024 Jun;176:171186. doi: 10.1016/j.peptides.2024.171186. Epub 2024 Mar 13.
Circulating insulin levels are known to be increased in people with higher body mass index (BMI) due to effects of adiposity on insulin resistance, whilst gut hormones have a more complex relationship, with fasting peptideYY (PYY) reported to be inversely related to BMI. This study aimed to further explore fasting and post prandial pancreatic and gut hormone concentrations in plasma samples from obese and non-obese participants. Participants with healthy BMI (n=15), overweight BMI (n=29) and obesity (n=161) had samples taken fasting and 30 min post mixed liquid meal for analysis of glucagon-like peptide-1 (GLP-1), PYY, glucose-dependent insulinotropic polypeptide (GIP), insulin and glucagon. Data visualiation used linear discriminant analysis for dimensionality reduction, to visualise the data and assess scaling of each hormone. Fasting levels of insulin, GIP and PYY were shown to be key classifiers between the 3 groups on ANCOVA analysis, with an observation of increased GIP levels in overweight, but not obese participants. In non-obese subjects, fasting GIP, PYY and insulin correlated with BMI, whereas in subjects with obesity only the pancreatic hormones glucagon and insulin correlated with BMI. Concentrations of total GLP-1 in the fasting state correlated strongly with glucagon levels, highlighting potential assay cross-reactivities. The study, which included a relatively large number of subjects with severe obesity, supported previous evidence of BMI correlating negatively with fasting PYY and positively with fasting insulin. The observation of increased fasting GIP levels in overweight but not obese participants deserves further validation and mechanistic investigation.
已知肥胖人群的体质量指数（BMI）较高，其循环胰岛素水平会升高，这是由于肥胖对胰岛素抵抗的影响所致，而肠道激素的关系则更为复杂，空腹肽 YY（PYY）与 BMI 呈负相关。本研究旨在进一步探讨肥胖和非肥胖参与者的空腹和餐后胰腺及肠道激素在血浆样本中的浓度。健康 BMI（n=15）、超重 BMI（n=29）和肥胖（n=161）的参与者在空腹和混合液体餐后 30 分钟采集样本，用于分析胰高血糖素样肽-1（GLP-1）、PYY、葡萄糖依赖性胰岛素释放肽（GIP）、胰岛素和胰高血糖素。线性判别分析用于数据可视化，以降低数据维度，评估每种激素的标度。ANCOVA 分析显示，空腹胰岛素、GIP 和 PYY 水平是 3 组之间的关键分类器，超重参与者的 GIP 水平升高，但肥胖参与者的 GIP 水平没有升高。在非肥胖受试者中，空腹 GIP、PYY 和胰岛素与 BMI 相关，而在肥胖受试者中，只有胰腺激素胰高血糖素和胰岛素与 BMI 相关。空腹状态下总 GLP-1 浓度与胰高血糖素水平高度相关，提示潜在的测定交叉反应。这项研究包括了大量的严重肥胖患者，支持了之前关于 BMI 与空腹 PYY 呈负相关、与空腹胰岛素呈正相关的证据。在超重但非肥胖参与者中观察到空腹 GIP 水平升高，这值得进一步验证和机制研究。
