Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Science, Jiangsu Normal University, Xuzhou, 221116, China.
The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, China.
BMC Plant Biol. 2024 Mar 16;24(1):193. doi: 10.1186/s12870-024-04824-z.
Sweetpotato (Ipomoea batatas (L.) Lam.) holds a crucial position as one of the staple foods globally, however, its yields are frequently impacted by environmental stresses. In the realm of plant evolution and the response to abiotic stress, the RNA helicase family assumes a significant role. Despite this importance, a comprehensive understanding of the RNA helicase gene family in sweetpotato has been lacking. Therefore, we conducted a comprehensive genome-wide analysis of the sweetpotato RNA helicase family, encompassing aspects such as chromosome distribution, promoter elements, and motif compositions. This study aims to shed light on the intricate mechanisms underlying the stress responses and evolutionary adaptations in sweetpotato, thereby facilitating the development of strategies for enhancing its resilience and productivity. 300 RNA helicase genes were identified in sweetpotato and categorized into three subfamilies, namely IbDEAD, IbDEAH and IbDExDH. The collinearity relationship between the sweetpotato RNA helicase gene and 8 related homologous genes from other species was explored, providing a reliable foundation for further study of the sweetpotato RNA helicase gene family's evolution. Furthermore, through RNA-Seq analysis and qRT-PCR verification, it was observed that the expression of eight RNA helicase genes exhibited significant responsiveness to four abiotic stresses (cold, drought, heat, and salt) across various tissues of ten different sweetpotato varieties. Sweetpotato transgenic lines overexpressing the RNA helicase gene IbDExDH96 were generated using A.rhizogenes-mediated technology. This approach allowed for the preliminary investigation of the role of sweetpotato RNA helicase genes in the response to cold stress. Notably, the promoters of RNA helicase genes contained numerous cis-acting elements associated with temperature, hormone, and light response, highlighting their crucial role in sweetpotato abiotic stress response.
甘薯（Ipomoea batatas (L.) Lam.）作为全球主要粮食作物之一，具有重要地位，但它的产量经常受到环境胁迫的影响。在植物进化和非生物胁迫响应中，RNA 解旋酶家族起着重要作用。尽管如此，对甘薯 RNA 解旋酶家族的全面了解仍有所欠缺。因此，我们对甘薯 RNA 解旋酶家族进行了全面的全基因组分析，包括染色体分布、启动子元件和基序组成等方面。本研究旨在揭示甘薯应对胁迫和进化适应的复杂机制，从而为提高其抗逆性和生产力提供策略。我们在甘薯中鉴定到 300 个 RNA 解旋酶基因，并将其分为三个亚家族：IbDEAD、IbDEAH 和 IbDExDH。我们还探索了甘薯 RNA 解旋酶基因与其他 8 个相关同源基因之间的共线性关系，为进一步研究甘薯 RNA 解旋酶基因家族的进化提供了可靠的基础。此外，通过 RNA-Seq 分析和 qRT-PCR 验证，我们观察到 8 个 RNA 解旋酶基因在 10 个不同甘薯品种的不同组织中对四种非生物胁迫（冷、干旱、热和盐）的表达具有显著的响应性。我们利用 A.rhizogenes 介导的技术生成了过表达 RNA 解旋酶基因 IbDExDH96 的甘薯转基因株系。该方法初步研究了甘薯 RNA 解旋酶基因在冷胁迫响应中的作用。值得注意的是，RNA 解旋酶基因的启动子包含许多与温度、激素和光响应相关的顺式作用元件，这表明它们在甘薯非生物胁迫响应中起着关键作用。
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