Wang Shuyu, Li Yuanlin, Yang Xiaoqi, Hao Yinxue, Zhan Xueyan
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Beijing Key Laboratory for Production Process Control and Quality Evaluation of Traditional Chinese Medicine, Beijing Municipal Science and Technology Commission, Beijing, China.
Front Pharmacol. 2024 Mar 1;15:1359954. doi: 10.3389/fphar.2024.1359954. eCollection 2024.
The purpose of this study was to identify the chemical components of Massa Medicata Fermentata (MMF) in different fermentation methods, analyze its regulatory effects on gastrointestinal propulsion and intestinal flora in mice with food accumulation, and further explore its mechanism of action in the treatment of dyspepsia. The chemical compositions of three kinds of MMF were identified using the UPLC-Q- Exactive Orbitrap mass spectrometer. A model of spleen deficiency and food accumulation in mice was established. The gastric emptying rate and intestinal propulsion rate were calculated, serum gastrin concentration and cholinesterase activity were measured, and 16S rRNA microbial detection was performed in different groups of mouse feces. The results showed that a total of 95 chemical components were identified from the three MMF extracts, 62 of which were the same, but there were differences in flavonoids and their glycosides, organic acids, and esters. MMF, PFMMF, and commercial MMF could all significantly improve the gastric emptying rate, intestinal propulsion rate, and GAS concentration in the serum of model mice; PFMMF has a better effect, while there was no significant difference in cholinesterase activity among the groups ( > 0.05). The 16S rRNA sequencing results showed that the MMF and PFMMF could increase the content of beneficial bacteria and decrease the pathogenic bacteria in the intestines of model mice, while the commercial MMF could not. Studies suggest that MMF has a variety of possible mechanisms for improving food accumulation and treating gastrointestinal dyspepsia, which provides reference value for the quality evaluation and clinical application of MMF.
本研究旨在鉴定不同发酵方法制备的六神曲的化学成分，分析其对食积小鼠胃肠推进及肠道菌群的调节作用，进一步探讨其治疗消化不良的作用机制。采用超高效液相色谱-四极杆-静电场轨道阱质谱仪鉴定三种六神曲的化学成分。建立小鼠脾虚食积模型，计算胃排空率和肠推进率，检测血清胃泌素浓度和胆碱酯酶活性，并对不同组小鼠粪便进行16S rRNA微生物检测。结果表明，从三种六神曲提取物中共鉴定出95种化学成分，其中62种相同，但黄酮类及其糖苷、有机酸和酯类存在差异。六神曲、炮制品六神曲和市售六神曲均能显著提高模型小鼠血清胃排空率、肠推进率和胃泌素浓度；炮制品六神曲效果更佳，各组胆碱酯酶活性差异无统计学意义（>0.05）。16S rRNA测序结果显示，六神曲和炮制品六神曲可增加模型小鼠肠道有益菌含量，减少致病菌，而市售六神曲则无此作用。研究表明，六神曲改善食积和治疗胃肠消化不良具有多种可能机制，为六神曲的质量评价和临床应用提供参考价值。
