Zhang Wenbin, Benke Ryan, Zhang Xiao, Zhang Huawen, Zhao Cunyuan, Zhao Yu, Xu Ying, Wang Hailian, Liu Shubing, Li Xianran, Wu Yuye
National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 China.
USDA-ARS, Wheat Health, Genetics, and Quality Research Unit, Pullman, WA 99164 USA.
Mol Breed. 2024 Mar 16;44(3):24. doi: 10.1007/s11032-024-01463-y. eCollection 2024 Mar.
Sorghum is an important food crop commonly used for brewing, feed, and bioenergy. Certain genotypes of sorghum contain high concentrations of condensed tannins in seeds, which are beneficial, such as protecting grains from herbivore bird pests, but also impair grain quality and digestibility. Previously, we identified and , each with three recessive causal alleles, regulate tannin absence in sorghum. In this study, via characterizing 421 sorghum accessions, we further identified three novel recessive alleles from these two genes. The allele contains a 12-bp deletion at position 659 nt and the allele contains a 10-bp deletion at position 771 nt in . The allele contains a C-to-T transition, which results in a premature stop codon before the bHLH domain in , and was predominantly selected in China. We further developed KASP assays targeting these identified recessive alleles to efficiently genotype large populations. These studies provide new insights in sorghum domestication and convenient tools for breeding programs.
The online version contains supplementary material available at 10.1007/s11032-024-01463-y.
高粱是一种重要的粮食作物，常用于酿造、饲料和生物能源。某些高粱基因型的种子中含有高浓度的缩合单宁，这既有益处，比如保护谷物免受食草鸟类害虫侵害，但也会损害谷物品质和消化率。此前，我们鉴定出 和 ，每个基因都有三个隐性因果等位基因，调控高粱中无单宁的性状。在本研究中，通过对421份高粱种质进行表征，我们从这两个基因中进一步鉴定出三个新的隐性等位基因。 等位基因在659 nt位置有一个12 bp的缺失， 等位基因在 的771 nt位置有一个10 bp的缺失。 等位基因发生了C到T的转换，导致在 中bHLH结构域之前出现提前终止密码子，并且在中国被广泛选择。我们进一步开发了针对这些已鉴定隐性等位基因的KASP检测方法，以高效地对大量群体进行基因分型。这些研究为高粱驯化提供了新见解，并为育种计划提供了便捷工具。
在线版本包含可在10.1007/s11032-024-01463-y获取的补充材料。
