Musculoskeletal Translational Innovation Initiative, Carl J. Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School; Mechanical Engineering Department, Boston University.
Musculoskeletal Translational Innovation Initiative, Carl J. Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School.
J Vis Exp. 2024 Mar 1(205). doi: 10.3791/65803.
Tendinopathy is a chronic tendon condition that results in pain and loss of function and is caused by repeated overload of the tendon and limited recovery time. This protocol describes a testing system that cyclically applies mechanical loads via passive dorsiflexion to the rat Achilles tendon. The custom-written code consists of pre- and post-cyclic loading measurements to assess the effects of the loading protocol along with the feedback control-based cyclic fatigue loading regimen. We used 25 Sprague-Dawley rats for this study, with 5 rats per group receiving either 500, 1,000, 2,000, 3,600, or 7,200 cycles of fatigue loads. The percentage differences between the pre- and post-cyclic loading measurements of the hysteresis, peak stress, and loading and unloading moduli were calculated. The results demonstrate that the system can induce varying degrees of damage to the Achilles tendon based on the number of loads applied. This system offers an innovative approach to apply quantified and physiological varying degrees of cyclic loads to the Achilles tendon for an in vivo model of fatigue-induced overuse tendon injury.
腱病是一种慢性腱病，导致疼痛和功能丧失，是由肌腱反复超负荷和有限的恢复时间引起的。本方案描述了一种通过被动背屈对大鼠跟腱周期性施加机械负荷的测试系统。自定义编写的代码包括预循环和后循环加载测量，以评估加载方案的效果以及基于反馈控制的周期性疲劳加载方案。我们使用 25 只 Sprague-Dawley 大鼠进行了这项研究，每组 5 只大鼠，分别接受 500、1000、2000、3600 或 7200 次疲劳负荷循环。计算了滞后、峰值应力以及加载和卸载模量的预循环和后循环加载测量之间的百分比差异。结果表明，该系统可以根据施加的负荷数量对跟腱造成不同程度的损伤。该系统为在体模型提供了一种创新的方法，可对跟腱施加量化和生理上不同程度的周期性负荷，以诱导疲劳性过度使用腱损伤。
