Yu Yue, Wang Siyue, Guo Wentong, Geng Meihui, Sun Ying, Li Wanjie, Yao Gaifang, Zhang Danfeng, Zhang Hua, Hu Kangdi
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China.
Plants (Basel). 2024 Feb 7;13(4):475. doi: 10.3390/plants13040475.
Hydrogen peroxide (HO) is relatively stable among ROS (reactive oxygen species) and could act as a signal in plant cells. In the present work, detached tomato leaves were treated with exogenous HO at 10 mmol/L for 8 h to study the mechanism of how HO regulates leaf senescence. The data indicated that HO treatment significantly accelerated the degradation of chlorophyll and led to the upregulation of the expression of leaf senescence-related genes (, , , , and ) during leaf senescence. HO treatment also induced the accumulation of HO and malondialdehyde (MDA), decreased POD and SOD enzyme activities and inhibited HS production by reducing the expression of and A correlation analysis indicated that HO was significantly and negatively correlated with chlorophyll, the expression of leaf senescence-related genes, and and . The principal component analysis (PCA) results show that HS showed the highest load value followed by O, HO, , , etc. Therefore, these findings provide a basis for studying the role of HO in regulating detached tomato leaf senescence and demonstrated that HO plays a positive role in the senescence of detached leaves by repressing antioxidant enzymes and HS production.
过氧化氢（H₂O₂）在活性氧（ROS）中相对稳定，并且可以在植物细胞中充当信号。在本研究中，将离体番茄叶片用10 mmol/L的外源H₂O₂处理8小时，以研究H₂O₂调节叶片衰老的机制。数据表明，H₂O₂处理显著加速了叶绿素的降解，并导致叶片衰老相关基因（，，，，和）在叶片衰老过程中的表达上调。H₂O₂处理还诱导了H₂O₂和丙二醛（MDA）的积累，降低了POD和SOD酶活性，并通过降低和的表达抑制了HS的产生。相关性分析表明，H₂O₂与叶绿素、叶片衰老相关基因的表达以及和显著负相关。主成分分析（PCA）结果表明，HS的载荷值最高，其次是O、H₂O₂、，，等。因此，这些发现为研究H₂O₂在调节离体番茄叶片衰老中的作用提供了依据，并证明H₂O₂通过抑制抗氧化酶和HS的产生在离体叶片衰老中起积极作用。
