Shenzhen Key Laboratory for Drug Addiction and Medication Safety, Department of Ultrasound, Institute of Ultrasonic Medicine, Peking University Shenzhen Hospital, The Hong Kong University of Science and Technology Medical Center, Shenzhen- Peking University, Shenzhen, P. R. China.
Department of Ultrasound, Nanjing Drum Tower Hospital, Nanjing, P. R. China.
PLoS One. 2024 Mar 18;19(3):e0289820. doi: 10.1371/journal.pone.0289820. eCollection 2024.
Inflammatory bowel disease (IBD) and atherosclerosis (AS) are both common chronic inflammatory diseases with similar pathophysiological mechanisms. Some studies have shown that IBD patients are at increased risk for early atherosclerosis, myocardial infarction and venous thrombosis. Here we set up a hybrid mouse model associated with atherosclerosis and acute colitis in order to investigate the interplay of the two diseases. We fed ApoE-/- mice with high fat diet to establish atherosclerosis model, and used animal ultrasound machine to detect the artery of mice noninvasively. Then a new hybrid model of atherosclerosis and acute colitis was prepared by drinking water for 7 days. At the end of the experiment, the hybrid model mice showed typically pathological and intuitionistic changes of atherosclerosis and acute colitis. We found the shortened colon length, high histopathological scores of the colon with mucosal erosion and necrosis, hyperlipidemia, a plaque-covered mouse aorta and plaque with foam cells and lipid deposition in the hybrid model group, which proved that the hybrid model was successfully established. At the same time, ultrasonic detection showed that the end-diastolic blood flow velocity and the relative dilation value were decreased, while systolic time / diastolic time, the wall thickness, systolic diameters as well as diastolic diameters were gradually increased, and statistical significance appeared as early as 8 weeks. We clearly described the process of establishing a hybrid model of atherosclerosis and acute colitis, which might provide a repeatable platform for the interaction mechanism exploring and drug screening of atherosclerosis and inflammatory bowel disease in preclinical study.
炎症性肠病（IBD）和动脉粥样硬化（AS）都是常见的慢性炎症性疾病，具有相似的病理生理机制。一些研究表明，IBD 患者发生早发动脉粥样硬化、心肌梗死和静脉血栓形成的风险增加。在这里，我们建立了一个与动脉粥样硬化和急性结肠炎相关的杂交小鼠模型，以研究这两种疾病的相互作用。我们用高脂肪饮食喂养 ApoE-/- 小鼠以建立动脉粥样硬化模型，并使用动物超声机对小鼠的动脉进行非侵入性检测。然后，通过连续 7 天饮水建立了新的动脉粥样硬化和急性结肠炎杂交模型。实验结束时，杂交模型小鼠表现出典型的动脉粥样硬化和急性结肠炎的病理和直观变化。我们发现，杂交模型组小鼠的结肠缩短，结肠黏膜糜烂和坏死的组织学评分较高，存在高脂血症、斑块覆盖的小鼠主动脉以及斑块中泡沫细胞和脂质沉积，证明该杂交模型成功建立。同时，超声检测显示，舒张末期血流速度和相对扩张值降低，而收缩时间/舒张时间、壁厚度、收缩直径以及舒张直径逐渐增加，早在 8 周时就出现了统计学意义。我们清楚地描述了建立动脉粥样硬化和急性结肠炎杂交模型的过程，为临床前研究中动脉粥样硬化和炎症性肠病的相互作用机制探索和药物筛选提供了一个可重复的平台。
