Guo Yan, Liu Xu-Cen, Wang Ya-Jie, Li Qi, Yang Qing, Weng Xiao-Gang, Chen Ying, Cai Wei-Yan, Kan Xiao-Xi, Chen Xi, Huang He-Fei, Zhu Xiao-Xin, Li Yu-Jie
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Dongzhimen Nei Nanxiao Road, Dongcheng District, Beijing, 100700, China.
BMC Complement Altern Med. 2016 Nov 15;16(1):469. doi: 10.1186/s12906-016-1449-6.
This study directly and dynamically investigated the effects of SL extract (i.e., a combination of Radix Salviae miltiorrhizae and Andrographis paniculata extract) on plaque progression in vivo by high resolution ultrasound biomicroscopy (UBM).
An atherosclerosis model was established by placing a perivascular collar on the right common carotid artery in apolipoprotein E-deficient (ApoE) mice. Thickness, plaque area and local blood flow were observed by UBM, pathological changes were observed by histochemical staining, and lipid levels were measured by respective commercially available kits.
Compared with the model group, the SL extract groups showed reduced wall thickness of the aortic arch (GC: P = 0.001, P = 0.002, and P < 0.001; LC: P < 0.001, P < 0.001, and P < 0.001; BC: P = 0.027, P = 0.017, and P = 0.003; respectively), which presented with retarded plaque progression of the cartoid artery with concordantly increased blood flow (P = 0.002 and P < 0.001) as visualized in vivo by UBM. Histological analysis confirmed the reduction of carotid atherosclerosis.
The SL extract inhibited the formation of atherosclerotic plaques in an ApoE mice model by UBM analysis, and did so by effects that ameliorated local blood flow and improved blood lipid levels.
本研究通过高分辨率超声生物显微镜（UBM）直接动态地研究了丹香提取物（即丹参和穿心莲提取物的组合）对体内斑块进展的影响。
通过在载脂蛋白E缺陷（ApoE）小鼠的右颈总动脉上放置血管周围套环建立动脉粥样硬化模型。通过UBM观察厚度、斑块面积和局部血流，通过组织化学染色观察病理变化，并使用相应的市售试剂盒测量血脂水平。
与模型组相比，丹香提取物组显示主动脉弓壁厚度降低（GC组：P = 0.001、P = 0.002和P < 0.001；LC组：P < 0.001、P < 0.001和P < 0.001；BC组：分别为P = 0.027、P = 0.017和P = 0.003），这表现为颈动脉斑块进展减缓，同时血流增加（P = 0.002和P < 0.001），通过UBM在体内可视化。组织学分析证实颈动脉粥样硬化减轻。
通过UBM分析，丹香提取物在ApoE小鼠模型中抑制了动脉粥样硬化斑块的形成，其作用机制是改善局部血流和血脂水平。
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