Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL, Canada.
Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL, Canada.
Dev Comp Immunol. 2024 Jul;156:105165. doi: 10.1016/j.dci.2024.105165. Epub 2024 Mar 16.
Renibacterium salmoninarum causes Bacterial Kidney Disease (BKD) in several fish species. Atlantic lumpfish, a cleaner fish, is susceptible to R. salmoninarum. To profile the transcriptome response of lumpfish to R. salmoninarum at early and chronic infection stages, fish were intraperitoneally injected with either a high dose of R. salmoninarum (1 × 10 cells dose) or PBS (control). Head kidney tissue samples were collected at 28- and 98-days post-infection (dpi) for RNA sequencing. Transcriptomic profiling identified 1971 and 139 differentially expressed genes (DEGs) in infected compared with control samples at 28 and 98 dpi, respectively. At 28 dpi, R. salmoninarum-induced genes (n = 434) mainly involved in innate and adaptive immune response-related pathways, whereas R. salmoninarum-suppressed genes (n = 1537) were largely connected to amino acid metabolism and cellular processes. Cell-mediated immunity-related genes showed dysregulation at 98 dpi. Several immune-signalling pathways were dysregulated in response to R. salmoninarum, including apoptosis, alternative complement, JAK-STAT signalling, and MHC-I dependent pathways. In summary, R. salmoninarum causes immune suppression at early infection, whereas lumpfish induce a cell-mediated immune response at chronic infection. This study provides a complete depiction of diverse immune mechanisms dysregulated by R. salmoninarum in lumpfish and opens new avenues to develop immune prophylactic tools to prevent BKD.
虹鳟鱼弧菌可引起多种鱼类的细菌性肾病（BKD）。清洁鱼大西洋庸鲽易感染虹鳟鱼弧菌。为了分析早期和慢性感染阶段庸鲽对虹鳟鱼弧菌的转录组应答，通过腹腔注射高剂量的虹鳟鱼弧菌（1×10 个细胞剂量）或 PBS（对照）对庸鲽进行处理。在感染后 28 天和 98 天分别采集头肾组织样本进行 RNA 测序。转录组谱分析鉴定出与对照组相比，在 28 天和 98 天 dpi 时，感染组分别有 1971 个和 139 个差异表达基因（DEGs）。在 28 天 dpi 时，虹鳟鱼弧菌诱导的基因（n=434）主要涉及先天和适应性免疫应答相关途径，而虹鳟鱼弧菌抑制的基因（n=1537）主要与氨基酸代谢和细胞过程有关。细胞介导的免疫相关基因在 98 天 dpi 时出现失调。几种免疫信号通路对虹鳟鱼弧菌的反应失调，包括细胞凋亡、替代补体、JAK-STAT 信号通路和 MHC-I 依赖性通路。总之，虹鳟鱼弧菌在早期感染时引起免疫抑制，而庸鲽在慢性感染时诱导细胞介导的免疫应答。本研究全面描绘了虹鳟鱼弧菌在庸鲽中失调的多种免疫机制，并为开发预防 BKD 的免疫预防工具开辟了新途径。
