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本土耐金属细菌物种金属吸收能力的评估——一项体外研究。

An assessment of metal absorption competence of indigenous metal tolerant bacterial species- an in-vitro study.

作者信息

Anusha P, Natarajan D, Rengarajan Sumathy, Alfarraj Saleh, Kandasamy Sabariswaran

机构信息

Department of Chemistry, Kongunadu College of Engineering and Technology, Thottiam Trichy- 621215, India.

Department of Biotechnology, Periyar University, Salem, 636 011, Tamil Nadu, India.

出版信息

Environ Res. 2024 Jun 15;251(Pt 2):118700. doi: 10.1016/j.envres.2024.118700. Epub 2024 Mar 16.

DOI:10.1016/j.envres.2024.118700
PMID:38499220
Abstract

Heavy metals pose a serious global threat to the environment. Hence, removing hazardous metals from soil samples has become complicated over the past few years. The current work looked into the remediation of heavy metals from aqueous solutions using a bacterial community and a unique bacterium obtained from metal-contaminated soil. In this investigation, the isolates of Bacillus anthracis A1-7, Bacillus. thuringiensis A1-3, Bacillus. cereus A1-5, and Pseudomonas aeruginosa A-33 actively demonstrated metal tolerances to various tested metals. Furthermore, an in-vitro biosorption study was performed under ideal concentration. The bacterial consortia achieved the highest biosorption effectiveness for Cu & Zn, 92.7% and 90.3%, respectively. When compared with a single bacterium, the group exhibited inferior Pb biosorption (86%). Since then, P. aeruginosa A33 has had the highest Pb biosorption. Finally, a bacterial consortium has devised an intriguing strategy for eliminating Cu and Pb from the polluted medium. P. aeruginosa A33 was found to be a mighty microbe that extracts Zn from polluted water. This metal-tolerant bacterium also exhibited specific proportions of selective commercially available antibiotics, which were analyzed using the Multiple Antibiotic Resistance (MAR) Index. In conclusion, these findings indicated that bacterial consortia composed of four bacterial isolates can remove metals from a metal-polluted medium.

摘要

重金属对全球环境构成严重威胁。因此,在过去几年中,从土壤样本中去除有害金属变得愈发复杂。当前的研究探讨了利用细菌群落和从金属污染土壤中分离出的一种独特细菌对水溶液中的重金属进行修复的方法。在这项调查中,炭疽芽孢杆菌A1 - 7、苏云金芽孢杆菌A1 - 3、蜡样芽孢杆菌A1 - 5和铜绿假单胞菌A - 33的分离菌株对各种测试金属均表现出积极的耐受性。此外,还在理想浓度下进行了体外生物吸附研究。细菌群落对铜和锌的生物吸附效率最高,分别达到92.7%和90.3%。与单一细菌相比,该群落对铅的生物吸附能力较差(86%)。从那时起,铜绿假单胞菌A33对铅的生物吸附能力最强。最后,一个细菌群落设计出了一种有趣的策略来从污染介质中去除铜和铅。发现铜绿假单胞菌A33是一种强大的微生物,能够从污染水中提取锌。这种耐金属细菌还表现出特定比例的对市售抗生素的选择性,这通过多重抗生素抗性(MAR)指数进行了分析。总之,这些发现表明,由四种细菌分离株组成的细菌群落可以从金属污染介质中去除金属。

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