细菌精氨酰 - 转移核糖核酸合成酶对三磷酸腺苷的消耗
Adenosine triphosphate consumption by bacterial arginyl-transfer ribonucleic acid synthetases.
作者信息
Godeau J M, Charlier J
出版信息
Biochem J. 1979 May 1;179(2):407-12. doi: 10.1042/bj1790407.
Abstract
ATP consumption by arginyl-tRNA synthetases from Escherichia coli and Bacillus stearothermophilus has been investigated by the firefly luciferin--luciferase assay. Arginyl-tRNA synthetase from E. coli utilizes ATP only for aminocylation of tRNA with a 1:1 stoicheiometry. In contrast, we have shown an adenosine triphosphatase activity of arginyl-tRNA synthetase from B. stearothermophilus in the absence of tRNAArg. Dowex chromatography revealed the formation of ADP by the thermophile enzyme; under aminoacylation conditions, AMP was also formed in amounts stoicheiometric with arginyl-tRNA formation.
摘要
通过萤火虫荧光素-荧光素酶测定法研究了来自大肠杆菌和嗜热脂肪芽孢杆菌的精氨酰-tRNA合成酶的ATP消耗情况。大肠杆菌的精氨酰-tRNA合成酶仅以1:1的化学计量比利用ATP进行tRNA的氨酰化。相比之下，我们已经证明在没有tRNAArg的情况下，嗜热脂肪芽孢杆菌的精氨酰-tRNA合成酶具有三磷酸腺苷酶活性。Dowex柱色谱显示嗜热菌酶形成了ADP；在氨酰化条件下，还形成了与精氨酰-tRNA形成化学计量相当的AMP。
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2
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Biochim Biophys Acta. 1963 Sep 17;76:48-53.
3
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