Center for Orthopedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, Ferdinand-Sauerbruch-Straße, 17475, Greifswald, Germany.
Department of General Pharmacology, Institute of Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine Greifswald, Felix-Hausdorff-Straße 3, 17489, Greifswald, Germany.
Sci Rep. 2024 Mar 18;14(1):6505. doi: 10.1038/s41598-024-56985-4.
Ewing's sarcoma (ES) is the second most common bone tumor in children and adolescents and is highly malignant. Although the new chemotherapy has significantly improved the survival rate for ES from about 10 to 75%, the survival rate for metastatic tumors remains around 30%. This treatment is often associated with various side effects that contribute to the suffering of the patients. Cold physical plasma (CPP), whether used alone or in combination with current chemotherapy, is considered a promising adjunctive tool in cancer treatment. This study aims to investigate the synergistic effects of CPP in combination with cytostatic chemotherapeutic agents that are not part of current ES therapy. Two different ES cell lines, RD-ES and A673, were treated with the determined IC concentrations of the chemotherapeutic agents cisplatin and methotrexate (MTX) in combination with CPP. The effects on population doubling, cell viability, and apoptotic processes within these cell lines were assessed. This combination therapy has led to a reduction of population doubling and cell viability, as well as an increase in apoptotic activity in cells compared to CPP monotherapy. The results of this study provide evidence that combining CPP with non-common chemotherapy drugs such as MTX and CIS in the treatment of ES enhances the anticancer effects of these drugs. These findings open up new possibilities for the effective use of these drugs against ES.
尤因氏肉瘤（Ewing's sarcoma，ES）是儿童和青少年中第二常见的骨肿瘤，具有高度恶性。尽管新的化疗方案显著提高了 ES 的生存率，从约 10%提高到 75%，但转移性肿瘤的生存率仍约为 30%。这种治疗常常伴有各种副作用，导致患者痛苦不堪。冷等离子体（Cold physical plasma，CPP），无论是单独使用还是与当前的化疗联合使用，都被认为是癌症治疗中一种有前途的辅助工具。本研究旨在探讨 CPP 与非当前 ES 治疗方案中的细胞毒性化疗药物联合使用的协同效应。使用确定的细胞毒性化疗药物顺铂（cisplatin）和氨甲蝶呤（methotrexate，MTX）的 IC 浓度，对两种不同的 ES 细胞系 RD-ES 和 A673 进行 CPP 处理。评估这些细胞系中群体倍增、细胞活力和凋亡过程的影响。与 CPP 单药治疗相比，这种联合治疗导致群体倍增和细胞活力减少，同时细胞凋亡活性增加。这项研究的结果提供了证据，表明 CPP 与 MTX 和 CIS 等非常见化疗药物联合用于 ES 的治疗可增强这些药物的抗癌作用。这些发现为有效利用这些药物对抗 ES 开辟了新的可能性。
