Laboratoire de Physiologie Cellulaire et Végétale, CNRS, CEA, INRAE, Univ. Grenoble Alpes, IRIG, CEA Grenoble, Grenoble, France.
Laboratoire de Physiologie Cellulaire et Végétale, IRIG, CEA-Grenoble, CNRS, CEA, INRAE, Univ. Grenoble Alpes, Grenoble, France.
Methods Mol Biol. 2024;2776:151-159. doi: 10.1007/978-1-0716-3726-5_8.
The outer and the inner membranes of the chloroplast envelope, also called OEM and IEM, have distinct lipid and protein compositions. They host molecular systems involved in the biogenesis of the organelle, its cellular function, and its communication with other compartments. Here we describe a method for the isolation of these two membranes starting from intact chloroplast preparations, with two alternative procedures based on the starting material. One was developed from spinach leaves, the other from pea leaves. The two procedures differ in the method used to isolate and rupture chloroplasts and separate each membrane.
叶绿体被膜的外膜和内膜，也被称为 OEM 和 IEM，具有不同的脂质和蛋白质组成。它们容纳了参与细胞器生物发生、细胞功能及其与其他隔室通讯的分子系统。在这里，我们描述了一种从完整的叶绿体制剂中分离这两种膜的方法，有两种基于起始材料的替代程序。一个是从菠菜叶片中开发的，另一个是从豌豆叶片中开发的。这两种程序在分离和破裂叶绿体以及分离每种膜的方法上有所不同。
