Department of Botany, University of Wisconsin, Madison, Wisconsin 53706.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3595-9. doi: 10.1073/pnas.78.6.3595.
A procedure for purifying the chloroplast envelope subfractionates it into two membrane fractions of comparable quantities. This procedure differs from previous ones in that the chloroplasts are ruptured by freezing and thawing in hypertonic medium rather than by osmotic shock. The two membrane fractions have qualitatively similar polar lipid compositions but differ in their content of individual lipids, specifically monogalactosyldiacylglycerol and phosphatidylcholine. The two fractions also differ in their constituent polypeptides and in their appearance when examined by electron microscopy. The light (density = 1.08 g/ml) and heavy (density = 1.13 g/ml) membrane fractions have been tentatively identified as the outer and inner envelope membranes, respectively.
一种分离叶绿体被膜亚组分的方法，它将叶绿体被膜分成两个数量相当的膜组份。该方法与以往的方法不同之处在于，叶绿体是通过在高渗介质中冷冻和融解而非渗透压冲击而被破坏。这两个膜组份的极性脂组成具有定性的相似性，但在个别脂类的含量上有所不同，特别是单半乳糖二酰基甘油和磷脂酰胆碱。这两个组份在其组成的多肽和电子显微镜观察的外观上也有所不同。轻（密度=1.08 g/ml）和重（密度=1.13 g/ml）膜组份已被初步鉴定为外被膜和内被膜。
Zotero 插件
