Department of Clinical Laboratory, Fuzhou Pulmonary Hospital and Fujian Medical University Clinical Teaching Hospital, Fuzhou, Fujian, China.
Department of Respiratory Medicine, Fuzhou Pulmonary Hospital and Fujian Medical University Clinical Teaching Hospital, Fuzhou, Fujian, China.
Crit Rev Immunol. 2024;44(4):41-49. doi: 10.1615/CritRevImmunol.2024052213.
Non-tuberculous mycobacteria (NTM) infection is common in bronchiectasis, with rising incidence globally. However, investigation into NTM in bronchiectasis patients in China remains relatively limited. This work aimed to identify and understand the features of NTM in bronchiectasis patient in Fuzhou district of China. The pulmonary samples were collected from 281 bronchiectasis patients with suspected NTM infection in Fuzhou, 2018-2022. MPB64 antigen detection was employed for the preliminary evaluation of NTM. Further NTM identification was realized using gene chip and gene sequencing. Among 281 patients, 172 (61.21%) patients were NTM-positive (58.72%) according to MPB64 antigen detection, with females (58.72%) outnumbering males (41.28%) and the highest prevalence in the age group of 46-65 years. In total, 47 NTM single infections and 3 mixed infections (1 Mycobacterium tuberculosis complex-M. intracellulare, 1 M. avium-M. intracellulare, and 1 M. abscessus-M. intracellulare) were identified through multicolor melting curve analysis (MMCA), which was compared with gene sequencing results. Both methods suggested Mycobacterium (M.) intracellulare, M. abscessus, and M. avium as the primary NTM species affecting bronchiectasis patients. M. intracellulare and M. abscessus were more frequent in females than males with the highest prevalence in the age group of 46-65 years according to MMCA. This research provides novel insights into the epidemiological and clinical features of NTM in bronchiectasis patients in Southeastern China. Significantly, M. intracellulare, M. abscessus, and M. avium were identified as the major NTM species, contributing to a better understanding and management of bronchiectasis accompanied by NTM infection.
非结核分枝杆菌（NTM）感染在支气管扩张症中很常见，全球发病率呈上升趋势。然而，中国对支气管扩张症患者中 NTM 的研究仍然相对有限。本研究旨在确定和了解中国福州地区支气管扩张症患者中 NTM 的特征。从 2018 年至 2022 年，从福州的 281 例疑似 NTM 感染的支气管扩张症患者中采集肺部样本。采用 MPB64 抗原检测对 NTM 进行初步评估。进一步通过基因芯片和基因测序对 NTM 进行鉴定。在 281 例患者中，根据 MPB64 抗原检测，有 172 例（61.21%）患者为 NTM 阳性（58.72%），女性（58.72%）多于男性（41.28%），年龄组中 46-65 岁的患病率最高。总共通过多色熔解曲线分析（MMCA）鉴定了 47 例 NTM 单一感染和 3 例混合感染（1 株结核分枝杆菌复合群-M. intracellulare、1 株 M. avium-M. intracellulare 和 1 株 M. abscessus-M. intracellulare），并与基因测序结果进行了比较。两种方法均提示分枝杆菌（M.）intracellulare、M. abscessus 和 M. avium 是影响支气管扩张症患者的主要 NTM 物种。根据 MMCA，M. intracellulare 和 M. abscessus 在女性中的发病率高于男性，在 46-65 岁年龄组中的发病率最高。本研究为中国东南部支气管扩张症患者 NTM 的流行病学和临床特征提供了新的见解。值得注意的是，M. intracellulare、M. abscessus 和 M. avium 被确定为主要的 NTM 物种，有助于更好地理解和管理伴有 NTM 感染的支气管扩张症。
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