National Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou 510642, China.
National Navel Orange Engineering Research Center, College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
J Agric Food Chem. 2024 Apr 3;72(13):6942-6953. doi: 10.1021/acs.jafc.3c09489. Epub 2024 Mar 20.
Thiamethoxam (THX), when applied to the soil, can be taken up by citrus roots and subsequently transported to the leaves, providing effective protection of plants against the Asian citrus psyllid ( Kuwayama). In this study, the field experiments showed that the coapplication of THX and nitrogen fertilizer (AN) did not affect THX uptake in six-year-old citrus plants. However, their coapplication promoted THX uptake in three-year-old rootstocks and relieved the inhibition of AN at a higher level on plant growth characteristics, including biomass and growth of root and stem. RNA-seq analysis found that THX induced upregulation of a cationic amino acid transporter () in citrus leaves. facilitated THX uptake in the yeast strain to inhibit its growth, and the PtCAT7 protein was localized on the plasma membrane. Our results demonstrate that THX and N fertilizer can be coapplied and may be involved in THX uptake in citrus.
噻虫嗪（THX）施用于土壤后，可被柑橘根系吸收，并随后转运至叶片，从而有效保护植物免受亚洲柑橘木虱（Kuwayama）的侵害。在本研究中，田间试验表明，噻虫嗪与氮肥（AN）共同施用不会影响 6 年生柑橘植株对噻虫嗪的吸收。然而，它们的共同施用促进了 3 年生砧木对噻虫嗪的吸收，并缓解了 AN 对植物生长特性（包括生物量和根、茎的生长）更高水平的抑制作用。RNA-seq 分析发现，噻虫嗪诱导柑橘叶片中阳离子氨基酸转运蛋白（）的上调。该基因在酵母菌株中促进噻虫嗪的摄取，抑制其生长，PtCAT7 蛋白定位于质膜上。我们的结果表明，噻虫嗪和氮肥可以共同施用，并且可能参与了柑橘对噻虫嗪的吸收。
