Institute of Industrial Biotechnology, Government College University, Lahore 54000, Pakistan.
Institute of Industrial Biotechnology, Government College University, Lahore 54000, Pakistan.
Int J Biol Macromol. 2024 Apr;265(Pt 1):130993. doi: 10.1016/j.ijbiomac.2024.130993. Epub 2024 Mar 19.
Hemicellulases are enzymes that hydrolyze hemicelluloses, common polysaccharides in nature. Thermophilic hemicellulases, derived from microbial strains, are extensively studied as natural biofuel sources due to the complex structure of hemicelluloses. Recent research aims to elucidate the catalytic principles, mechanisms and specificity of hemicellulases through investigations into their high-temperature stability and structural features, which have applications in biotechnology and industry. This review article targets to serve as a comprehensive resource, highlighting the significant progress in the field and emphasizing the vital role of thermophilic hemicellulases in eco-friendly catalysis. The primary goal is to improve the reliability of hemicellulase enzymes obtained from thermophilic bacterial strains. Additionally, with their ability to break down lignocellulosic materials, hemicellulases hold immense potential for biofuel production. Despite their potential, the commercial viability is hindered by their high enzyme costs, necessitating the development of efficient bioprocesses involving waste pretreatment with microbial consortia to overcome this challenge.
半纤维素酶是水解半纤维素的酶，半纤维素是自然界中常见的多糖。嗜热半纤维素酶来源于微生物菌株，由于半纤维素的复杂结构，被广泛研究为天然生物燃料来源。最近的研究旨在通过研究其高温稳定性和结构特征，阐明半纤维素酶的催化原理、机制和特异性，这些研究在生物技术和工业中有应用。本文综述旨在作为一个全面的资源，突出该领域的重要进展，并强调嗜热半纤维素酶在环保催化中的重要作用。主要目标是提高从嗜热细菌菌株获得的半纤维素酶的可靠性。此外，由于它们能够分解木质纤维素材料，半纤维素酶在生物燃料生产方面具有巨大的潜力。尽管有潜力，但由于酶的成本高，商业可行性受到限制，因此需要开发涉及微生物群落预处理废物的高效生物工艺来克服这一挑战。
