Department of Pediatrics, Medical School, University of Minnesota, Minneapolis, Minnesota.
Research Animal Resources, University of Minnesota, Minneapolis, Minnesota;, Email:
Comp Med. 2024 Apr 1;74(2):99-104. doi: 10.30802/AALAS-CM-23-000087. Epub 2024 Mar 20.
Preweaning mortality is a widespread problem in laboratory mouse breeding, particularly in the case of fragile mouse models. While numerous studies explore alternative care methods to increase the survivability of common mouse strains, there remains a paucity of research into the care of mice with fragile health conditions that result from induced or natural genetic mutations. In this study, standard husbandry practices were enhanced by the addition of a softened diet, a nutritionally fortified dietary supplement, soft bedding, gentle handling techniques, decreased handling, lengthened weaning age, and dam productivity tracking. This alternative care plan was shown to increase the survival of a fragile recessive dystrophic epidermolysis bullosa mouse model, and some aspects could be used in developing a care plan for other fragile mouse strains.
新生期死亡率是实验室小鼠繁殖中普遍存在的问题，尤其是在脆弱的小鼠模型中。虽然有许多研究探索了替代护理方法来提高常见小鼠品系的存活率，但对于因诱导或自然遗传突变而导致健康状况脆弱的小鼠的护理研究仍然很少。在这项研究中，通过添加软化饮食、营养强化膳食补充剂、柔软的床上用品、轻柔的处理技术、减少处理、延长断奶年龄和母鼠生产力跟踪，增强了标准的饲养实践。这种替代护理方案被证明可以提高脆弱隐性营养不良性大疱性表皮松解症小鼠模型的存活率，并且某些方面可以用于制定其他脆弱小鼠品系的护理计划。
