Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital of Jinan University, Guangzhou, Guangdong, China.
Key Laboratory of Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, Guangdong, China.
J Cell Mol Med. 2024 Apr;28(7):e18236. doi: 10.1111/jcmm.18236.
A three-dimensional alginate-coated scaffold (GAIS) was constructed in the present study to showcase the multidifferentiation potential of peripheral blood mesenchymal stem cells (PBMSCs) and to investigate the role and mechanism by which Icariin (ICA)/stromal cell-derived factor (SDF-1α)/PBMSCs promote damaged articular repair. In addition, the ability of ICA, in combination with SDF-1α, to promote the migration and proliferation of stem cells was validated through the utilization of CCK-8 and migration experiments. The combination of ICA and SDF-1α inhibited the differentiation of PBMSCs into cartilage, as demonstrated by in vivo experiments and histological staining. Both PCR and western blot experiments showed that GAIS could upregulate the expression of particular genes in chondrocytes. In comparison to scaffolds devoid of alginate (G0), PBMSCs seeded into GAIS scaffolds exhibited a greater rate of proliferation, and the conditioned medium derived from scaffolds containing SDF-1α enhanced the capacity for cell migration. Moreover, after a 12-week treatment period, GAIS, when successfully transplanted into osteochondral defects of mice, was found to promote cartilage regeneration and repair. The findings, therefore, demonstrate that GAIS enhanced the in vitro capabilities of PBMSCs, including proliferation, migration, homing and chondrogenic differentiation. In addition, ICA and SDF-1α effectively collaborated to support cartilage formation in vivo. Thus, the ICA/SDF-1α/PBMSC-loaded biodegradable alginate-gelatin scaffolds showcase considerable potential for use in cartilage repair.
本研究构建了一种三维海藻酸钙涂层支架（GAIS），展示了外周血间充质干细胞（PBMSCs）的多向分化潜能，并探讨了淫羊藿苷（ICA）/基质细胞衍生因子（SDF-1α）/PBMSCs 促进受损关节修复的作用和机制。此外，通过 CCK-8 实验和迁移实验验证了 ICA 与 SDF-1α 联合促进干细胞迁移和增殖的能力。体内实验和组织学染色表明，ICA 和 SDF-1α 的联合抑制了 PBMSCs 向软骨的分化。PCR 和 Western blot 实验均表明 GAIS 可上调软骨细胞中特定基因的表达。与不含海藻酸钠的支架（G0）相比，接种到 GAIS 支架上的 PBMSCs 增殖速度更快，且含有 SDF-1α 的支架的条件培养基增强了细胞迁移能力。此外，经过 12 周的治疗期后，成功移植到小鼠的骨软骨缺损中的 GAIS 被发现可促进软骨再生和修复。因此，这些发现表明 GAIS 增强了 PBMSCs 的体外能力，包括增殖、迁移、归巢和软骨分化。此外，ICA 和 SDF-1α 有效协作，支持体内软骨形成。因此，负载 ICA/SDF-1α/PBMSC 的可生物降解海藻酸钙-明胶支架在软骨修复中具有很大的应用潜力。
