Zhang Ju, Zhao Yuying, Peng Zhaoxin, Yang MingFei, Zou Wenyu, Wu Xinyu, Wang Chenghui, Si Meiru, Chen Can
Key Laboratory of Plant Genetics and Molecular Breeding, Henan Key Laboratory of Crop Molecular Breeding & Bioreactor, College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, Henan, China.
College of Horticulture, Agricultural University of Hebei/Key Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei/Collaborative Innovation Center of Vegetable Industry in Hebei, Baoding, 071001, China.
Heliyon. 2024 Mar 10;10(6):e27929. doi: 10.1016/j.heliyon.2024.e27929. eCollection 2024 Mar 30.
The gene () of encodes a repressor of the TetR (tetracycline regulator) family. Its role in the stress response to antibiotics/heavy metals has been investigated, but how CssR functions in response to phenolic compounds in has been rarely studied. In this study, we applied transcriptomic analysis, β-galactosidase analysis, qRT-PCR, and EMSAs to analyze the target genes and functions of CssR in response to phenolic compounds. Consistent with the upregulation of genes involved in the degradation of phenolic compounds, the Δ mutant was more resistant to various phenolic compounds than was the wild-type strain. Furthermore, the addition of phenolic compounds induced the expression of corresponding genes (, , , , , and ) . However, the DNA binding activity of CssR to the promoter of phenolic compound-degrading genes was undetected . Additionally, we also found that CssR indirectly negatively regulates the expression of cell wall/membrane/envelope biogenesis-related genes, which may enhance resistance to stress caused by phenolic compounds. Together, our findings demonstrate that CssR is a key regulator that copes with stress conditions induced by phenolic compounds, thus greatly expanding our understanding of the functions of TetR family transcription factors.
编码一种TetR（四环素调节因子）家族的阻遏物。其在对抗生素/重金属应激反应中的作用已被研究，但CssR在应对中的酚类化合物时如何发挥作用却鲜有研究。在本研究中，我们应用转录组分析、β-半乳糖苷酶分析、qRT-PCR和电泳迁移率变动分析（EMSA）来分析CssR响应酚类化合物的靶基因和功能。与参与酚类化合物降解的基因上调一致，Δ突变体比野生型菌株对各种酚类化合物更具抗性。此外，添加酚类化合物诱导了相应基因（、、、、和）的表达。然而，未检测到CssR与酚类化合物降解基因启动子的DNA结合活性。此外，我们还发现CssR间接负调控细胞壁/膜/包膜生物合成相关基因的表达，这可能增强对酚类化合物引起的应激的抗性。总之，我们的研究结果表明CssR是应对酚类化合物诱导的应激条件的关键调节因子，从而极大地扩展了我们对TetR家族转录因子功能的理解。
