预测和致癌作用的坏死相关的 miR-425-5p 和 miR-16-5p 在食管鳞癌。
Predictive and Carcinogenic Roles of Necroptosis-Related miR-425-5p and miR-16-5p in Esophageal Squamous Cell Carcinoma.
机构信息
Department of Oncology Radiotherapy, the Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
Department of Oncology Radiotherapy, the Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China
出版信息
Ann Clin Lab Sci. 2024 Jan;54(1):76-85.
OBJECTIVE
To examine the expression and function of necroptosis-associated miRNAs in esophageal squamous cell carcinoma.
METHODS
A total of three microarray datasets, i.e., GSE122497, GSE114110, and GSE43732, were selected from the GEO database for differential analysis of necroptosis-related miRNA expression. The differentially expressed miRNAs were screened for target miRNAs using Kaplan-Meier survival analysis in the OncomiR database. The expression of the target miRNAs in the HEEC, KYSE-450, TE-1, and KYSE-410 cell lines was measured via qPCR. The expression of the target miRNAs in esophageal cancer cells was regulated by transfection with Lipofectamine 2000, and cell proliferation, cell migration, cell apoptosis and the cell cycle were detected by CCK-8, Transwell, and flow cytometry.
RESULTS
The tumor tissue and peripheral blood of esophageal squamous cell cancer patients showed differential expression of 7 miRNAs related to necroptosis. Survival analysis revealed that miR-425-5p and miR-16-5p were negatively correlated with patient survival. The esophageal squamous cell carcinoma cell lines exhibited increased expression of miR-425-5p and miR-16-5p, with KYSE-410 exhibiting the most significant increase. Inhibition of miR-425-5p and miR-16-5p expression in the KYSE-410 cell line resulted in increased apoptosis, decreased proliferation, and decreased migration of esophageal cancer cells as well as a significant increase in the S phase and a decrease in the G2/M phase according to the cell cycle assay.
CONCLUSION
The pro-carcinogenic role of miR-425-5p and miR-16-5p has been observed in esophageal squamous cell carcinoma.
目的
研究食管鳞癌细胞中坏死相关 miRNA 的表达和功能。
方法
从 GEO 数据库中选择了三个微阵列数据集（GSE122497、GSE114110 和 GSE43732），用于对坏死相关 miRNA 表达进行差异分析。使用 OncomiR 数据库中的 Kaplan-Meier 生存分析筛选差异表达 miRNA 的靶 miRNA。通过 qPCR 测量靶 miRNA 在 HEEC、KYSE-450、TE-1 和 KYSE-410 细胞系中的表达。通过 Lipofectamine 2000 转染调节食管癌细胞中靶 miRNA 的表达，通过 CCK-8、Transwell 和流式细胞术检测细胞增殖、细胞迁移、细胞凋亡和细胞周期。
结果
食管癌患者肿瘤组织和外周血显示与坏死相关的 7 种 miRNA 存在差异表达。生存分析表明 miR-425-5p 和 miR-16-5p 与患者生存呈负相关。食管鳞癌细胞系中 miR-425-5p 和 miR-16-5p 的表达增加，其中 KYSE-410 增加最明显。在 KYSE-410 细胞系中抑制 miR-425-5p 和 miR-16-5p 的表达导致食管癌细胞凋亡增加、增殖减少、迁移减少，细胞周期检测显示 S 期显著增加，G2/M 期减少。
结论
在食管鳞癌中观察到 miR-425-5p 和 miR-16-5p 的致癌作用。
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