Bedi G S, Back N
Prep Biochem. 1985;15(3):159-69. doi: 10.1080/10826068508062269.
Monoclonal antibody to rat plasma kininogen, obtained after immunization of mice with the kininogen prepared by conventional methods, was purified from ascites fluid and coupled to CNBr-activated Sepharose-4B. Monoclonal-antibody affinity adsorbant thus prepared provided a rapid single-step method of purifying to homogeneity plasma kininogen. Purified rat plasma kininogen showed identical molecular weight and immunological cross-reactivity to rat plasma low molecular weight (LMW) kininogen purified by conventional procedures. Rat plasma kininogen differed from LMW kininogen from other species by virtue of its resistance to cleavage by either plasma or glandular kallikreins.
用常规方法制备的激肽原免疫小鼠后获得的抗大鼠血浆激肽原单克隆抗体，从腹水液中纯化并偶联到溴化氰活化的琼脂糖-4B上。由此制备的单克隆抗体亲和吸附剂提供了一种快速的单步方法，可将血浆激肽原纯化至同质。纯化的大鼠血浆激肽原与通过常规方法纯化的大鼠血浆低分子量（LMW）激肽原具有相同的分子量和免疫交叉反应性。大鼠血浆激肽原与其他物种的LMW激肽原不同，因为它对血浆或腺体激肽释放酶的切割具有抗性。
