Endalew Sisay Awoke, Taddese Minbale Gashu, Muhammed Meseret
Chemistry Department, College of Natural Sciences Wollo University Dessie Ethiopia.
Chemistry Department, College of Natural and Computational Sciences Debre Berhan University Debre Berhan Ethiopia.
Health Sci Rep. 2024 Mar 21;7(3):e1990. doi: 10.1002/hsr2.1990. eCollection 2024 Mar.
, an endemic plant to Ethiopia, traditionally used to treat infectious as well as noninfectious diseases. The primary objective of this study was isolating dehydrocostus lactone (DHCL) from and evaluating antibacterial activities on selected human pathogenic bacteria.
Extraction method used in this study was maceration. Based on the bioassay information methanol extract of the root of was subjected to column chromatography on silica gel by increasing solvent gradients to isolate DHCL. Optimized amount isolation of DHCL was done by dissolving methanol crude extract by hexane followed by recrystallization at room temperature in the dark place. Different concentrations of the extract were subjected by disc diffusion method against tested bacterial species and antioxidant activity test.
The phytochemical analysis of revealed a high presence of terpenoids, which are diverse natural compounds known for their antimicrobial and antioxidant properties. This suggests that terpenoids contribute significantly to the pharmacological effects of . In antibacterial testing, was the most sensitive bacterium among all extracts and concentrations. The methanol extract displayed higher antioxidant activity compared to ethyl acetate and hexane extracts, indicating a higher concentration of antioxidant compounds. Notably, the isolated compound DHCL showed promising activity against tested pathogens and significant antioxidant activity. The higher activity of DHCL compared to the crude extracts suggests its responsibility for the observed effects, indicating that the isolation and purification process may have concentrated its beneficial properties. These findings highlight the potential of and DHCL as sources of bioactive compounds for therapeutic applications.
All tested extracts and pure compound showed higher inhibition than positive controls in both bioassay. DHCL the principal bioactive component in the root extract of the plant and it displayed potent antibacterial and antioxidant activity.
[植物名称]是埃塞俄比亚的一种本土植物，传统上用于治疗传染病和非传染病。本研究的主要目的是从[植物名称]中分离脱氢木香内酯（DHCL），并评估其对选定的人类病原菌的抗菌活性。
本研究采用的提取方法是浸渍法。根据生物测定信息，[植物名称]根的甲醇提取物通过硅胶柱色谱法，采用递增溶剂梯度来分离DHCL。通过用己烷溶解甲醇粗提物，然后在室温黑暗处重结晶来优化DHCL的分离量。不同浓度的提取物通过纸片扩散法对受试细菌进行测试，并进行抗氧化活性测试。
[植物名称]的植物化学分析表明，萜类化合物含量很高，萜类化合物是一类以其抗菌和抗氧化特性而闻名的多种天然化合物。这表明萜类化合物对[植物名称]的药理作用有显著贡献。在抗菌测试中，[受试细菌名称]在所有提取物和浓度中对[植物名称]最敏感。与乙酸乙酯和己烷提取物相比，甲醇提取物显示出更高的抗氧化活性，表明抗氧化化合物浓度更高。值得注意的是，分离出的化合物DHCL对受试病原体显示出有前景的活性和显著的抗氧化活性。与粗提物相比，DHCL的活性更高，表明其对观察到的效果有作用，这表明分离和纯化过程可能浓缩了其有益特性。这些发现突出了[植物名称]和DHCL作为治疗应用生物活性化合物来源的潜力。
在两种生物测定中，所有测试的提取物和纯化合物均显示出比阳性对照更高的抑制作用。DHCL是该植物根提取物中的主要生物活性成分，它显示出强大的抗菌和抗氧化活性。
