Durden Leigh, Eckhoff Kyle, Burdsall Adam C, Youn Sungmin, Andújar-Gonzalez Cindy, Abu-Niaaj Lubna, Magnuson Matthew, Harper Willie F
Department of Systems Engineering and Management, Engineering Management Program, Air Force Institute of Technology, 2950 Hobson Way, Wright-Patterson AFB, OH, USA.
Water Infrastructure Protection Division, National Homeland Security Research Center, US Environmental Protection Agency, Cincinnati, Ohio, USA.
Environ Sci (Camb). 2023 Nov 8;9(12):3458-3466. doi: 10.1039/d3ew00524k.
This study characterized (BG) as a Sterne (BAS) surrogate for wastewater treatment-related studies of UV inactivation, adsorption onto powdered activated carbon (PAC), and bioaerosol emission. The inactivation of BG was faster than that of BAS in DI water (pseudo first-order rate constants of 0.065 and 0.016 min respectively) and in PBS solution (0.030 and 0.005 min respectively). BG was also removed more quickly than BAS by PAC adsorption in DI (0.07 and 0.05 min respectively) and in PBS (0.09 and 0.04 min respectively). In DI, BG aggregated more ( < 0.05) than BAS when the pH was 7 or greater but there were no statistically significant differences in NaCl solution. Spore aggregation was also studied with extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) models. Less than 1% of all spores were released as bioaerosols, and there was no significant difference ( > 0.05) in emission between BG and BAS. To the author's knowledge, this study is the first to demonstrate that BG is a suitable surrogate for BAS for bioaerosol emissions, but a poor surrogate for both UV inactivation and PAC adsorption. These results can be used to understand the ability of BAS to act as a surrogate for BA Ames because of its genetic and morphological similarities with BAS.
本研究将芽孢杆菌属（BG）作为斯特恩芽孢杆菌（BAS）的替代物，用于紫外线灭活、吸附到粉末活性炭（PAC）以及生物气溶胶排放等与废水处理相关的研究。在去离子水（伪一级反应速率常数分别为0.065和0.016 min）和磷酸盐缓冲溶液（PBS，分别为0.030和0.005 min）中，BG的灭活速度比BAS快。在去离子水（分别为0.07和0.05 min）和PBS（分别为0.09和0.04 min）中，BG通过PAC吸附去除的速度也比BAS快。在去离子水中，当pH值为7或更高时，BG比BAS聚集得更多（p<0.05），但在氯化钠溶液中没有统计学上的显著差异。还使用扩展的Derjaguin-Landau-Verwey-Overbeek（XDLVO）模型研究了孢子聚集情况。所有孢子中释放为生物气溶胶的不到1%，BG和BAS之间的排放没有显著差异（p>0.05）。据作者所知，本研究首次证明BG是BAS在生物气溶胶排放方面的合适替代物，但在紫外线灭活和PAC吸附方面是较差的替代物。由于BG与BAS在遗传和形态上的相似性，这些结果可用于理解BAS作为BA艾姆斯替代物的能力。
