Stankiewicz M, Jonas W
Arch Immunol Ther Exp (Warsz). 1985;33(3):423-8.
Based on conglutination tests with the sheep E-rabbit A indicator system, three types of sheep sera were encountered. Type 1 sera failed to directly conglutinate or sensitize sheep E-rabbit A for conglutination by bovine conglutinin. Type 2 sera also failed to directly conglutinate sheep E-rabbit A but sensitized the indicator for conglutination by bovine conglutinin. Type 3 sera both directly conglutinated and sensitized sheep E-rabbit A for conglutination. Changes in serum type were induced in sheep by venepuncture (type 1 to type 2) or venepuncture and an intraperitoneal injection of yeast cells (type 2 to type 3). Direct conglutinating activity of type 3 sera was inhibited by heating serum at 50 degrees C for 30 min and was not restored by alternative activation pathway factor B. Chelation of Ca2+ in type 2 and 3 sera blocked sensitization of sheep E-rabbit A for conglutination by bovine conglutinin, indicating that the classical activation pathway was involved.
基于使用绵羊E-兔A指示系统的凝集试验，发现了三种类型的绵羊血清。1型血清不能直接凝集或致敏绵羊E-兔A以便被牛凝集素凝集。2型血清也不能直接凝集绵羊E-兔A，但能致敏该指示物以便被牛凝集素凝集。3型血清既能直接凝集又能致敏绵羊E-兔A以便被凝集。通过静脉穿刺（1型转变为2型）或静脉穿刺并腹腔注射酵母细胞（2型转变为3型）可使绵羊血清类型发生改变。3型血清的直接凝集活性在50℃加热血清30分钟后被抑制，且不能通过替代激活途径因子B恢复。2型和3型血清中Ca2+的螯合阻断了绵羊E-兔A被牛凝集素凝集的致敏作用，表明涉及经典激活途径。
