绵羊血清凝集补体活性的变化以及与兔抗体致敏的绵羊红细胞反应所涉及的途径。
Variations in sheep serum conglutinating complement activity and pathway involved in reactivity with sheep erythrocytes sensitized by rabbit antibody.
作者信息
Stankiewicz M, Jonas W
出版信息
Arch Immunol Ther Exp (Warsz). 1985;33(3):423-8.
Based on conglutination tests with the sheep E-rabbit A indicator system, three types of sheep sera were encountered. Type 1 sera failed to directly conglutinate or sensitize sheep E-rabbit A for conglutination by bovine conglutinin. Type 2 sera also failed to directly conglutinate sheep E-rabbit A but sensitized the indicator for conglutination by bovine conglutinin. Type 3 sera both directly conglutinated and sensitized sheep E-rabbit A for conglutination. Changes in serum type were induced in sheep by venepuncture (type 1 to type 2) or venepuncture and an intraperitoneal injection of yeast cells (type 2 to type 3). Direct conglutinating activity of type 3 sera was inhibited by heating serum at 50 degrees C for 30 min and was not restored by alternative activation pathway factor B. Chelation of Ca2+ in type 2 and 3 sera blocked sensitization of sheep E-rabbit A for conglutination by bovine conglutinin, indicating that the classical activation pathway was involved.
基于使用绵羊E-兔A指示系统的凝集试验，发现了三种类型的绵羊血清。1型血清不能直接凝集或致敏绵羊E-兔A以便被牛凝集素凝集。2型血清也不能直接凝集绵羊E-兔A，但能致敏该指示物以便被牛凝集素凝集。3型血清既能直接凝集又能致敏绵羊E-兔A以便被凝集。通过静脉穿刺（1型转变为2型）或静脉穿刺并腹腔注射酵母细胞（2型转变为3型）可使绵羊血清类型发生改变。3型血清的直接凝集活性在50℃加热血清30分钟后被抑制，且不能通过替代激活途径因子B恢复。2型和3型血清中Ca2+的螯合阻断了绵羊E-兔A被牛凝集素凝集的致敏作用，表明涉及经典激活途径。
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