Qi Fang-Yuan, Qiao Lei, Peng Lan, Yang Yu, Zhang Chong-Hua, Liu Xianjun
School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China.
Central Laboratory of the Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University, Xuzhou First People's Hospital, Xuzhou 221116, Jiangsu, China.
Analyst. 2024 Apr 15;149(8):2299-2305. doi: 10.1039/d4an00188e.
Nitroxyl (HNO) plays a vital role in various biological functions and pharmacological activities, so the development of an excellent near-infrared fluorescent (NIRF) and photoacoustic (PA) dual-modality probe is crucial for understanding HNO-related physiological and pathological progression. Herein, we proposed and synthesized a novel NIRF/PA dual probe (QL-HNO) by substituting an indole with quinolinium in hemicyanine for the sensitive detection of exogenous and endogenous HNO . The designed probe showed the highest sensitivity in NIRF mode and a desirable PA signal-to-noise ratio for HNO detection and was further applied for NIRF/PA dual-modal imaging of HNO with high contrast in living cells and tumor-bearing animals. Based on the excellent performance of QL-HNO, we believe that this study provides a promising molecular tool for further understanding of HNO-related physiological and pathological progression.
硝酰（HNO）在各种生物学功能和药理活性中起着至关重要的作用，因此开发一种出色的近红外荧光（NIRF）和光声（PA）双模态探针对于理解与HNO相关的生理和病理进展至关重要。在此，我们通过用喹啉鎓取代半菁中的吲哚，提出并合成了一种新型的NIRF/PA双探针（QL-HNO），用于灵敏检测外源性和内源性HNO。所设计的探针在NIRF模式下表现出最高的灵敏度，并且对于HNO检测具有理想的PA信噪比，并进一步应用于活细胞和荷瘤动物中具有高对比度的HNO的NIRF/PA双模态成像。基于QL-HNO的优异性能，我们相信这项研究为进一步理解与HNO相关的生理和病理进展提供了一种有前景的分子工具。
