Meiniel A, Meiniel R
Exp Biol. 1985;44(3):191-8.
The glycoproteins of the outer segment membrane of photoreceptor cells in the pineal organ of Lampetra planeri were studied using the affined properties of lectins to sugar moieties. Among five different lectins, labelled with fluorescein-isothiocyanate, Concanavalin A (Con A) and wheat germ agglutinin (WGA) induced an intense fluorescence on the large outer segments protruding into the lumen of the pineal organ. The complementary sugars, alpha-D-mannopyranosyl (for Con A) and N-acetyl-D-glucosamine (for WGA), inhibit the binding of both lectins. By means of electron microscopy, after incubation in Con A and labelling with peroxidase, and intense enzymatic activity was revealed in the diskal spaces by the technique of Graham and Karnovsky (1966). These results suggest the presence of glycoproteins rich in mannose and N-acetyl-D-glucosamine thus implying the presence of a photopigment of similar composition and structure to that of rhodopsin or rhodopsin itself.
利用凝集素对糖部分的亲和特性，研究了溪七鳃鳗松果体中光感受器细胞外段膜的糖蛋白。在五种用异硫氰酸荧光素标记的不同凝集素中，刀豆球蛋白A（Con A）和麦胚凝集素（WGA）在突出到松果体腔中的大外段上诱导出强烈的荧光。其互补糖，α-D-吡喃甘露糖基（针对Con A）和N-乙酰-D-葡萄糖胺（针对WGA），抑制这两种凝集素的结合。通过电子显微镜，在用Con A孵育并用过氧化物酶标记后，采用Graham和Karnovsky（1966年）的技术，在盘状间隙中显示出强烈的酶活性。这些结果表明存在富含甘露糖和N-乙酰-D-葡萄糖胺的糖蛋白，因此意味着存在与视紫红质组成和结构相似的光色素或视紫红质本身。
