海洋硬骨鱼中从受体水平和延髓水平获得的对氨基酸的嗅觉反应比较。

Comparison of the olfactory responses to amino acids obtained from receptor and bulbar levels in a marine teleost.

作者信息

Kobayashi H, Goh Y

出版信息

Exp Biol. 1985;44(3):199-210.

Abstract

Comparison of the olfactory responses to amino acids was made between electro-olfactogram (EOG) and induced bulbar electroencephalogram (EEG) in a marine teleost, red sea bream (Pargus major). The increase of the magnitude of EOG responses to five amino acids (L-glutamine, L-alanine, L-methionine, L-phenylalanine and L-aspartic acid) was shown to have exponential functions against logarithmic increase of the stimulus concentration within the range tested (10(-5) to 10(-2) M), and were found to have similar exponents ranging from 0.21 to 0.25. In EEG, dose-response curves for six amino acids (L-glutamine, L-alanine, L-arginine, L-methionine, L-serine and glycine) showed two different functions within the range tested (10(-5) to 10(-2) M). Against logarithmic increase of the stimulus concentration, responses increased exponentially for L-glutamine and L-arginine, and sigmoidally for L-alanine, L-methionine, L-serine and glycine, and even in the exponential functions exponents showed considerable diversity (0.12 to 0.21). Comparison of relative stimulatory effectiveness of amino acids between the two responses (EOG vs. EEG) revealed that in general they are similar. But a considerable difference was also found in L-glutamic acid which was more effective in producing EOG than EEG.

摘要

在一种海洋硬骨鱼——真鲷（Pargus major）中，比较了嗅电图（EOG）和诱发的延髓脑电图（EEG）对氨基酸的嗅觉反应。在测试范围内（10⁻⁵至10⁻²M），对五种氨基酸（L-谷氨酰胺、L-丙氨酸、L-甲硫氨酸、L-苯丙氨酸和L-天冬氨酸）的EOG反应幅度的增加显示出与刺激浓度的对数增加呈指数函数关系，并且发现其指数相似，范围从0.21至0.25。在EEG中，六种氨基酸（L-谷氨酰胺、L-丙氨酸、L-精氨酸、L-甲硫氨酸、L-丝氨酸和甘氨酸）的剂量-反应曲线在测试范围内（10⁻⁵至10⁻²M）显示出两种不同的函数关系。随着刺激浓度的对数增加，L-谷氨酰胺和L-精氨酸的反应呈指数增加，而L-丙氨酸、L-甲硫氨酸、L-丝氨酸和甘氨酸的反应呈S形增加，并且即使在指数函数中，指数也显示出相当大的差异（0.12至0.21）。两种反应（EOG与EEG）之间氨基酸相对刺激效果的比较表明，总体上它们是相似的。但在L-谷氨酸中也发现了相当大的差异，L-谷氨酸在产生EOG方面比EEG更有效。