The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA; Departments of Surgery, Biochemistry, and Molecular Medicine, Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
STAR Protoc. 2024 Jun 21;5(2):102973. doi: 10.1016/j.xpro.2024.102973. Epub 2024 Mar 21.
The characterization of cell populations that reside in the outer layer of the heart has been hindered by difficulties in their isolation. Here, we present a protocol for isolation and single-nuclei multiomic analyses of the human fetal epicardium. We describe steps for microdissection, isolation, and enrichment of epicardial cells by mechanical dissociations and direct lysis. We then detail procedures for integrating transcriptome and chromatin accessibility datasets. This approach allows the analysis of diverse cell populations, marked by unique cis-regulatory elements. For complete details on the use and execution of this protocol, please refer to Travisano et al..
对存在于心脏外层的细胞群体进行特征描述一直受到其分离困难的阻碍。在这里，我们提出了一种分离和单细胞多组学分析人类胎儿心外膜的方案。我们描述了通过机械解离和直接裂解进行心外膜细胞的显微解剖、分离和富集的步骤。然后，我们详细介绍了整合转录组和染色质可及性数据集的程序。这种方法允许分析具有独特顺式调控元件的不同细胞群体。有关此方案使用和执行的完整详细信息，请参阅 Travisano 等人的研究。
