Department of Neurosurgery, Binzhou Medical University Hospital, Binzhou, China.
The First School of Clinical Medicine of Binzhou Medical University, Binzhou, China.
Medicine (Baltimore). 2024 Mar 22;103(12):e37523. doi: 10.1097/MD.0000000000037523.
Previous research has indicated that the rupture of intracranial aneurysm (IA) is a significant contributor to mortality from stroke. The objective of this present study was to examine the infiltration patterns in ruptured intracranial aneurysm (RIA), with the aim of generating insights that could inform the development of effective immunotherapeutic approaches.
To achieve this, we obtained Gene Expression Omnibus datasets pertaining to ruptured aneurysms, encompassing a total of 19 unruptured intracranial aneurysms (UIA) and 27 RIA. Subsequently, we conducted differential gene analysis and immune cell analysis specifically for the RIA.
According to the conducted studies, the analysis has identified 10 hub genes within key modules. Through the utilization of Kyoto Encyclopedia of Genes and Genomes pathway and gene ontology terms analyses, it has been established that genes exhibiting differential expression are associated with immune cell infiltration in the aneurysm wall. Furthermore, the implementation of the CIBERSORT algorithm has revealed that there are 22 distinct immune cells between RIA and tissues of UIA. IA samples contained a higher proportion of macrophages M1, mast cells resting, and CD4 naive T cells, while macrophages M0 and neutrophils were relatively lower in RIA compared with those in UIA.
The current study initially identified highly conservative hub genes and immune cell infiltration patterns in IA. Data presented in the current study improved understanding of immune genes that drive IA which can be exploited in development of effective immunotherapies.
先前的研究表明，颅内动脉瘤（IA）破裂是导致中风死亡的一个重要因素。本研究旨在研究破裂颅内动脉瘤（RIA）的浸润模式，旨在为开发有效的免疫治疗方法提供思路。
为此，我们获得了与破裂动脉瘤相关的基因表达综合数据集，其中包括 19 个未破裂颅内动脉瘤（UIA）和 27 个 RIA。然后，我们对 RIA 进行了差异基因分析和免疫细胞分析。
根据进行的研究，分析确定了关键模块中的 10 个枢纽基因。通过京都基因与基因组百科全书通路和基因本体术语分析，确定了差异表达的基因与动脉瘤壁中免疫细胞浸润有关。此外，使用 CIBERSORT 算法表明，RIA 和 UIA 组织之间存在 22 种不同的免疫细胞。IA 样本中巨噬细胞 M1、静止肥大细胞和 CD4 幼稚 T 细胞的比例较高，而 M0 巨噬细胞和中性粒细胞的比例较低。
本研究首次鉴定了 IA 中高度保守的枢纽基因和免疫细胞浸润模式。本研究中提供的数据增进了对驱动 IA 的免疫基因的理解，这可以用于开发有效的免疫疗法。
