Bioprocess and Bioenergy Laboratory (BPBEL), Department of Microbiology, Central University of Rajasthan, Bandarsindri, Kishangarh, Ajmer, Rajasthan, 305817, India.
Algal Technology Program, Center for Sustainable Development, College of Arts and Sciences, Qatar University, Doha, 2713, Qatar.
Environ Pollut. 2024 May 1;348:123796. doi: 10.1016/j.envpol.2024.123796. Epub 2024 Mar 20.
Municipal wastewater (MWW) was treated by a sequential pilot microalgal cultivation process. The cultivation was performed inside a specifically designed low-cost photobioreactor (PBR) system. A microalgal consortium 2:1 was developed using Tetraselmis indica (TS) and Picochlorum sp. (PC) in the first stage and PC:TS (2:1) in the second stage and the nutrient removal efficiency and biomass production and biomolecules production was evaluated and also compared with monoculture in a two-stage sequential cultivation system. This study also investigated the effect of seasonal variations on microalgae growth and MWW treatment. The results showed that mixed microalgal consortium (TS:PC) had higher nutrient removal efficiency, with chemical oxygen demand (COD), total phosphate (TP), and total nitrate (TN) removal efficiencies of 78.50, 84.49, and 84.20%, respectively, and produced a biomass of 2.50 g/L with lipid content of 37.36% in the first stage of cultivation under indoor conditions. In the second stage of indoor cultivation, the PC:TS consortium demonstrated maximum COD, TP, and TN removal efficiencies of 92.49, 94.24, and 94.16%, respectively. It also produced a biomass of 2.65 g/L with a lipid content of 40.67%. Among all the seasonal variations, mass flow analysis indicated that the combination of mixed consortium-based two-stage sequential process during the winter season favored maximum nutrient removal efficiency of TN i.e. 88.54% (84.12 mg/L) and TP i.e., 90.18% (43.29 mg/L), respectively. It also enhanced total biomass production of 49.10 g in 20-L medium, which includes lipid yield ∼15.68 g compared to monoculture i.e., 82.06% (78.70 mg/L) and 82.87% (40.26 mg/L) removal of TN and TP, respectively, and produced biomass 43.60 g with 11.90 g of lipids.
采用序批式微藻培养工艺处理城市污水（MWW）。培养在专门设计的低成本光生物反应器（PBR）系统中进行。在第一阶段使用三角褐指藻（TS）和微绿球藻（PC）开发了微藻共生体 2:1，在第二阶段使用 PC:TS（2:1），并评估了营养物质去除效率、生物量生产和生物分子生产，并与两阶段序批式培养系统中的单培养进行了比较。本研究还调查了季节性变化对微藻生长和 MWW 处理的影响。结果表明，混合微藻共生体（TS:PC）具有更高的营养物质去除效率，室内条件下第一阶段培养的化学需氧量（COD）、总磷酸盐（TP）和总硝酸盐（TN）去除效率分别为 78.50%、84.49%和 84.20%，生物量为 2.50 g/L，脂质含量为 37.36%。在室内培养的第二阶段，PC:TS 共生体表现出最大的 COD、TP 和 TN 去除效率，分别为 92.49%、94.24%和 94.16%。它还产生了 2.65 g/L 的生物量，脂质含量为 40.67%。在所有季节性变化中，质量流量分析表明，冬季混合共生体两阶段序批式工艺的结合有利于 TN 的最大营养物质去除效率，即 88.54%（84.12mg/L）和 TP，即 90.18%（43.29mg/L）。它还在 20-L 培养基中提高了 49.10g 的总生物量产量，其中包括脂质产量约 15.68g，与单培养相比，TN 和 TP 的去除率分别为 82.06%（78.70mg/L）和 82.87%（40.26mg/L），生物量为 43.60g，脂质为 11.90g。
