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整合蛋白质组学和代谢组学数据分析探索慢性应激大鼠心脏手术后脑损伤的机制。

Integrative proteomics and metabolomics data analysis exploring the mechanism of brain injury after cardiac surgery in chronic stress rats.

机构信息

Department of Anesthesiology, Fuwai Hospital, National Center of Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, People's Republic of China.

Department of Cardiology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.

出版信息

BMC Anesthesiol. 2024 Mar 22;24(1):111. doi: 10.1186/s12871-024-02492-y.

DOI:10.1186/s12871-024-02492-y
PMID:38519946
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10958840/
Abstract

OBJECTIVE

Preoperative chronic stress (CS) is associated with postoperative brain injury in patients undergoing open heart cardiac surgery. This research is to explore the potential molecular biological mechanisms of brain damage following cardiac surgery in preoperative CS rats by the analyses combining proteomics and metabolomics.

METHODS

We constructed the chronic unpredictable stress (CUS) and cardiac surgery models in adult rats. We proved the brain injury in CUS cardiac surgery rats by Hematoxylin-Eosin (H&E) staining, followed by separating the hippocampal tissue and investigating the potential mechanisms of brain injury by the methods of data-independent acquisition proteomics and untargeted metabolomics.

RESULTS

The signaling pathways of glycoproteins and metabolism of amino acids were the main possible mechanisms of brain injury in CUS rats following cardiac surgery according to the proteomics and metabolomics. In addition, the pathways of animo acids metabolism such as the pathways of lysine degradation and β-alanine metabolism may be the main mechanism of cardiac surgery related brain injury in preoperative CUS rats.

CONCLUSIONS

The pathways of animo acids metabolism such as lysine degradation and β-alanine metabolism may be the potential mechanisms of brain injury in CUS rats following cardiac surgery. We should focus on the varieties of bioproteins and metabolites in these pathways, and related changes in other signaling pathways induced by the two pathways.

摘要

目的

术前慢性应激（CS）与接受心脏直视手术的患者术后脑损伤有关。本研究通过蛋白质组学和代谢组学相结合的分析，探讨术前 CS 大鼠心脏手术后脑损伤的潜在分子生物学机制。

方法

我们构建了成年大鼠的慢性不可预测应激（CUS）和心脏手术模型。通过苏木精-伊红（H&E）染色证实 CUS 心脏手术大鼠的脑损伤，然后分离海马组织，通过非靶向代谢组学和数据非依赖性采集蛋白质组学的方法研究脑损伤的潜在机制。

结果

根据蛋白质组学和代谢组学，糖蛋白信号通路和氨基酸代谢可能是 CUS 大鼠心脏手术后脑损伤的主要可能机制。此外，氨基酸代谢途径，如赖氨酸降解和β-丙氨酸代谢途径，可能是术前 CUS 大鼠心脏手术相关脑损伤的主要机制。

结论

氨基酸代谢途径，如赖氨酸降解和β-丙氨酸代谢途径，可能是 CUS 大鼠心脏手术后脑损伤的潜在机制。我们应关注这些途径中的生物蛋白和代谢物的变化，以及这两条途径诱导的其他信号通路的相关变化。

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