Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmaceutical Sciences, The Hashemite University, Zarqa, Jordan.
Department of Biology and Biotechnology, Faculty of Science, The Hashemite University, Zarqa, Jordan.
Inflammopharmacology. 2024 Jun;32(3):2035-2048. doi: 10.1007/s10787-024-01449-4. Epub 2024 Mar 23.
The aim of this investigation was to explore the protective impacts and mechanisms of Anastatica hierochuntica essential oil (EOAH) against dextran sulfate sodium (DSS)-induced experimental colitis in mice. EOAH demonstrated a reduction in DSS-induced body weight decline, disease activity index (DAI), colon length reduction, colonic tissue damage, and myeloperoxidase (MPO) activity. The essential oil significantly mitigated the production of pro-inflammatory agents including TNF-α, IL-1β, and IL-12. Further analysis revealed that EOAH's anti-inflammatory effects involved the regulation of NF-κB and PPARγ pathways, as well as the inhibition of NLRP3 activation in colitis mice. Notably, EOAH treatment elevated the levels of beneficial commensal bacteria such as Lactobacillus and Bifidobacteria, while reducing Escherichia coli levels in the mice's feces. In addition, EOAH restored the expression of occludin and ZO-1 proteins in colonic tissues affected by ulcerative colitis (UC). These findings indicate that supplementing with EOAH might offer a novel therapeutic approach for UC prevention.
本研究旨在探讨播娘蒿挥发油（EOAH）对葡聚糖硫酸钠（DSS）诱导的实验性结肠炎小鼠的保护作用及其机制。EOAH 可降低 DSS 诱导的体重下降、疾病活动指数（DAI）、结肠缩短、结肠组织损伤和髓过氧化物酶（MPO）活性。该精油还可显著减轻促炎因子 TNF-α、IL-1β 和 IL-12 的产生。进一步的分析表明，EOAH 的抗炎作用涉及 NF-κB 和 PPARγ 途径的调节，以及对结肠炎小鼠 NLRP3 激活的抑制。值得注意的是，EOAH 治疗可提高有益共生菌（如乳酸菌和双歧杆菌）的水平，同时降低小鼠粪便中的大肠杆菌水平。此外，EOAH 还可恢复溃疡性结肠炎（UC）影响的结肠组织中闭合蛋白和 ZO-1 蛋白的表达。这些发现表明，补充 EOAH 可能为 UC 的预防提供一种新的治疗方法。
