Department of Chemistry, Faculty of Science, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia.
Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia.
J Fish Dis. 2024 Jul;47(7):e13940. doi: 10.1111/jfd.13940. Epub 2024 Mar 24.
Vibrio alginolyticus is the causative agent of vibriosis, a common bacterial infection in grouper aquaculture that is associated with the development of haemorrhagic and non-haemorrhagic ulcerations on the fish. In the present study, comparative proteome analysis was performed on serum samples from Vibrio-resistant and Vibrio-susceptible grouper. Samples were analysed using high-throughput LC-MS/MS and identified 2770 unique peptides that corresponded to 344 proteins. Subsequent analysis identified 21 proteins that were significantly up-regulated in the resistant group compared to the control and the susceptible groups. Those proteins are associated with immunostimulatory effects, signalling and binding cascade, metabolism, and maintaining tissue integrity and physiological condition. Besides, potential protein biomarkers related to the immune system were identified, which could be associated with the disease-resistant phenotype. These data provide insights into the underlying immune mechanism of hybrid groupers upon Vibrio sp. infection.
哈氏弧菌是弧菌病的病原体，弧菌病是一种常见的鱼类细菌性疾病，可导致石斑鱼出现出血性和非出血性溃疡。在本研究中，对耐哈氏弧菌和易感哈氏弧菌石斑鱼的血清样本进行了比较蛋白质组学分析。使用高通量 LC-MS/MS 对样本进行分析，鉴定出 2770 个独特肽段，对应 344 种蛋白质。进一步的分析鉴定出 21 种在耐药组中显著上调的蛋白，与对照组和易感组相比。这些蛋白与免疫刺激作用、信号转导和结合级联、代谢以及维持组织完整性和生理状态有关。此外，还鉴定出与免疫系统相关的潜在蛋白生物标志物，这些标志物可能与抗病表型有关。这些数据为哈氏弧菌感染杂交石斑鱼的潜在免疫机制提供了深入了解。
