School of Dentistry, Stomatological Hospital, Tianjin Medical University, Tianjin, China.
Department of Stomatology, Tianjin First Central Hospital, Tianjin, China.
Head Neck. 2024 Jun;46(6):1450-1467. doi: 10.1002/hed.27742. Epub 2024 Mar 24.
The aim was to research ACTL6A's role in oral squamous cell carcinoma (OSCC).
OSCC and normal samples were obtained from patients and public databases. GSEA was performed. CIBERSORT was utilized to analyze immune landscape. Kaplan-Meier survival analysis and multivariate Cox regression analysis were conducted. After knocking down ACTL6A, we performed MTT assay, transwell assays, and flow cytometry to detect the impact of knockdown.
ACTL6A expressed higher in OSCC samples than normal samples. The CNV and mutation rate of TP53 was higher in ACTL6A high-expression group. TFs E2F7 and TP63 and miRNA hsa-mir-381 were significantly related to ACTL6A. ACTL6A could influence immune microenvironment of OSCC. Knockdown of ACTL6A inhibited OSCC cells' proliferation, migration, and invasion. ACTL6A was able to predict OSCC prognosis independently.
ACTL6A expressed higher in OSCC than normal samples and it could be used as an independent prognostic marker in OSCC patients.
研究 ACTL6A 在口腔鳞状细胞癌（OSCC）中的作用。
从患者和公共数据库中获取 OSCC 和正常样本。进行 GSEA。使用 CIBERSORT 分析免疫景观。进行 Kaplan-Meier 生存分析和多变量 Cox 回归分析。敲低 ACTL6A 后，进行 MTT 检测、transwell 检测和流式细胞术，以检测敲低的影响。
ACTL6A 在 OSCC 样本中的表达高于正常样本。ACTL6A 高表达组中 TP53 的 CNV 和突变率更高。TFs E2F7 和 TP63 以及 miRNA hsa-mir-381 与 ACTL6A 显著相关。ACTL6A 可影响 OSCC 的免疫微环境。敲低 ACTL6A 抑制 OSCC 细胞的增殖、迁移和侵袭。ACTL6A 可独立预测 OSCC 预后。
ACTL6A 在 OSCC 中的表达高于正常样本，可作为 OSCC 患者的独立预后标志物。
