Ahmad Ibrar, Khan Muhammad Nadeem, Hayat Khizar, Ahmad Tanveer, Shams Dilawar Farhan, Khan Waliullah, Tirth Vineet, Rehman Gauhar, Muhammad Wazir, Elhadi Muawya, Alotaibi Afraa, Shah Said Karim
Department of Basic and Applied Sciences for Engineering, Sapienza University of Rome, Rome 00185, Italy.
Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, Khyber Pakhtunkhwa, Pakistan.
ACS Omega. 2024 Mar 9;9(11):13208-13216. doi: 10.1021/acsomega.3c09851. eCollection 2024 Mar 19.
Silver nanoparticles (Ag-NPs) were synthesized by using the polyol method. The structural and morphological characteristics of Ag-NPs were studied by using X-ray diffraction (XRD) and field-emission scanning electron microscopy (FE-SEM). The XRD analysis revealed the formation of single-phase polycrystalline Ag-NPs with an average crystallite size and lattice constant of ∼23 nm and 4.07 Å, respectively, while the FE-SEM shows the formation of a uniform and spherical morphology. Energy-dispersive X-ray spectroscopy confirmed the formation of single-phase Ag-NPs, and no extra elements were detected. A strong absorption peak at ∼427 nm was observed in the UV-vis spectrum, which reflects the surface plasmon resonance (SPR) behavior characteristic of Ag-NPs with a spherical morphology. Fourier-transform infrared (FTIR) spectra also supported the XRD and EDX results with regard to the purity of the prepared Ag-NPs. Anti-inflammatory activity was tested using HRBCs membrane stabilization and heat-induced hemolysis assays. The antibacterial activity of Ag-NPs was evaluated against four different types of pathogenic bacteria by using the disc diffusion method (DDM). The Gram-negative bacterial strains used in this study are (), Klebsiella, Shigella, and Salmonella. The analysis suggested that the antibacterial activities of Ag-NPs have an influential role in inhibiting the growth of the tested Gram-negative bacteria, and thus Ag-NPs can find a potential application in the pharmaceutical industry.
采用多元醇法合成了银纳米颗粒（Ag-NPs）。利用X射线衍射（XRD）和场发射扫描电子显微镜（FE-SEM）研究了Ag-NPs的结构和形态特征。XRD分析表明形成了单相多晶Ag-NPs，平均晶粒尺寸和约23 nm，晶格常数约为4.07 Å，而FE-SEM显示形成了均匀的球形形态。能量色散X射线光谱证实了单相Ag-NPs的形成，未检测到额外元素。在紫外可见光谱中观察到约427 nm处有一个强吸收峰，这反映了具有球形形态的Ag-NPs的表面等离子体共振（SPR）行为特征。傅里叶变换红外（FTIR）光谱也支持了关于所制备Ag-NPs纯度的XRD和EDX结果。使用人红细胞（HRBCs）膜稳定化和热诱导溶血试验测试了抗炎活性。采用纸片扩散法（DDM）评估了Ag-NPs对四种不同类型病原菌的抗菌活性。本研究中使用的革兰氏阴性细菌菌株为（）、克雷伯菌、志贺菌和沙门氏菌。分析表明，Ag-NPs的抗菌活性在抑制受试革兰氏阴性细菌的生长中具有重要作用，因此Ag-NPs在制药工业中具有潜在应用价值。
