Kusmiati Kusmiati, Fanani Asrul, Nurkanto Arif, Purnaningsih Ismu, Mamangkey Jendri, Ramadhani Indriati, Nurcahyanto Dian Alfian, Simanjuntak Partomuan, Afiati Fifi, Irawan Herman, Puteri Ade Lia, Ewaldo Muhammad Farrel, Juanssilfero Ario Betha
Research Center for Biosystematics and Evolution- Research Organization for Life Sciences and Environment, The National Research and Innovation Agency (BRIN), Indonesia.
Research and Education Center for Bioinformatics, Indonesia Institute of Bioinformatics, Malang, 65162, Indonesia.
Heliyon. 2024 Mar 11;10(6):e27978. doi: 10.1016/j.heliyon.2024.e27978. eCollection 2024 Mar 30.
Endophytic fungi are known for producing secondary metabolites with valuable biological activities, including antiviral, anticancer, antibacterial, and antioxidant properties. This study aims to evaluate an endophytic fungus from Dragon Scales leaves () and analyze its metabolites as antioxidants and antibacterials. In this study, an endophytic fungus was isolated from the leaves of Dragon Scales (. ) and identified using molecular analysis of the Internal Transcribed Spacer (ITS) ribosomal RNA locus. The fungus was authenticated as strain K-10. Crude extracts were obtained using -hexane and ethyl acetate and analyzed via GC-MS Shimadzu-QP 2010 Ultra with NIST spectral library. Antibacterial activity was observed against and using the paper disc method, showing inhibition zones of 8.7-9.3 mm and 8.8-9.4 mm for ethyl acetate and -hexane extracts, respectively. Ethyl acetate and -hexane extracts exhibited strong antioxidant potential against 2,2-diphenyl-1-picrylhydrazil (DPPH) radical (IC values of 50.99 μg mL and 74.44 μg mL, respectively). GC-MS analysis revealed 40 compounds in both extracts, some of which, including 2-ethylhexyl ester benzoic acid, benzo-b-dihydropyran-6-hydroxy-4-4-5-7-8-pentamethyl, diethyl phthalate, and octadecanoic acid, were identified through analysis and found to possess antioxidant properties. These findings hold implications for potential applications of the plant and its biological constituent to be developed as lead compounds in the medical sector.
内生真菌以产生具有重要生物活性的次生代谢产物而闻名，这些生物活性包括抗病毒、抗癌、抗菌和抗氧化特性。本研究旨在评估来自龙鳞叶（）的一种内生真菌，并分析其作为抗氧化剂和抗菌剂的代谢产物。在本研究中，从龙鳞叶（. ）中分离出一种内生真菌，并通过对内部转录间隔区（ITS）核糖体RNA基因座进行分子分析来鉴定。该真菌被鉴定为菌株K-10。使用正己烷和乙酸乙酯获得粗提取物，并通过配备NIST光谱库的岛津QP 2010 Ultra气相色谱-质谱联用仪进行分析。采用纸片法观察到对和的抗菌活性，乙酸乙酯提取物和正己烷提取物的抑菌圈分别为8.7 - 9.3毫米和8.8 - 9.4毫米。乙酸乙酯提取物和正己烷提取物对2,2-二苯基-1-苦基肼（DPPH）自由基表现出较强的抗氧化潜力（IC值分别为50.99微克/毫升和74.44微克/毫升）。气相色谱-质谱分析在两种提取物中均揭示了40种化合物，其中一些化合物，包括2-乙基己基苯甲酸酯、苯并- b -二氢吡喃-6-羟基-4,4,5,7,8-五甲基、邻苯二甲酸二乙酯和十八烷酸，通过分析鉴定出来并发现具有抗氧化特性。这些发现对于该植物及其生物成分在医学领域作为先导化合物进行开发的潜在应用具有重要意义。
