Exploring the therapeutic potential of pomegranate juice for uterine relaxation.

BACKGROUND

The effects of pomegranate juice (PJ) and its components on uterine smooth muscle are unknown. Hence, this study unequivocally demonstrates that pomegranate juice (PJ) significantly impacts myometrial function, providing crucial insights into its relaxant properties and their potential therapeutic applications for uterine-related disorders.

RESEARCH DESIGN AND METHODS

Rat uterine smooth muscle horn strips were suspended in Krebs solution organ baths. Contractions were measured isometrically using a transducer (AD instrument Australia). The effects of PJ were evaluated on contractile activity elicited by potassium chloride (KCl 60 Mm) depolarization. Inhibitors of nitric oxide (L-NAME 3 X 10), guanylate cyclase (methylene blue 1 X 10), and Prostaglandin I (indomethacin 3 X 10), as well as Potassium Channels blockers, were determined.

RESULTS

The juice at concentrations from 1.5-5 mg/ml significantly decreased the rat uterine horn contraction induced by KCl. The NO, cGMP, and PGI inhibitors did not block the relaxation response. Furthermore, the PGI inhibitor significantly enhanced the relaxation effects; K channel blockers had no inhibitory effects on the relaxation responses. Contrarily, GLIB improved considerably relaxation.

CONCLUSION

Research suggests pomegranate juice's active ingredient may reduce uterine contractions and treat uterotonic disorders, potentially preventing preterm birth and dysmenorrhea. Further research is needed to determine its mechanism of action.

TRIAL REGISTRATION

Code: AEC-013.