Lin Ling, Hao Zhigang, Zhou Liying, Liu Wenyi, Liu Nian, Wang Kebao, Jia Ruizong
Sanya Research Institution/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Chinese Academy of Tropical Agriculture Sciences, Sanya, China.
Foshan Lianyi Biotechnology Co., Ltd., Foshan, China.
Mitochondrial DNA B Resour. 2024 Mar 20;9(3):371-375. doi: 10.1080/23802359.2024.2329674. eCollection 2024.
Roxb., a plant species of significant pharmaceutical interest, has been the subject of limited chloroplast genomic research. In this study, we present the sequencing and assembly of the chloroplast genome, which is characterized by a circular chromosome spanning 162,212 base pairs and a GC content of 36.20%. The genome encodes 87 protein-coding genes (PCGs), 38 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. A phylogenetic analysis was conducted, incorporating eight related species, and based on the complete chloroplast genome and protein-coding DNA sequences of six related taxa within the genus. Outgroup species and were also included in the analysis. The results indicate a close relationship between and , , and . This study provides the first chloroplast genome of , thereby contributing a valuable genomic resource for future research on medicinal plants within the genus.
罗汉果，一种具有重要药用价值的植物物种，一直是叶绿体基因组研究较少的对象。在本研究中，我们展示了叶绿体基因组的测序和组装，其特征是一个环状染色体，跨度为162,212个碱基对，GC含量为36.20%。该基因组编码87个蛋白质编码基因（PCG）、38个转运RNA（tRNA）基因和8个核糖体RNA（rRNA）基因。进行了系统发育分析，纳入了8个相关物种，并基于该属内6个相关分类群的完整叶绿体基因组和蛋白质编码DNA序列。外类群物种和也被纳入分析。结果表明与、和之间关系密切。本研究提供了罗汉果的首个叶绿体基因组，从而为该属药用植物的未来研究贡献了宝贵的基因组资源。
