Laboratory of Biophysical Chemistry, Kyoto Pharmaceutical University, Japan.
Center for Instrumental Analysis, Kyoto Pharmaceutical University, Japan.
FEBS Lett. 2024 Apr;598(8):902-914. doi: 10.1002/1873-3468.14858. Epub 2024 Mar 26.
Apolipoprotein E (apoE) is a regulator of lipid metabolism, cholesterol transport, and the clearance and aggregation of amyloid β in the brain. The three human apoE isoforms apoE2, apoE3, and apoE4 only differ in one or two residues. Nevertheless, the functions highly depend on the isoform types and lipidated states. Here, we generated novel anti-apoE monoclonal antibodies (mAbs) and obtained an apoE4-selective mAb whose epitope is within residues 110-117. ELISA and bio-layer interferometry measurements demonstrated that the dissociation constants of mAbs are within the nanomolar range. Using the generated antibodies, we successfully constructed sandwich ELISA systems, which can detect all apoE isoforms or selectively detect apoE4. These results suggest the usability of the generated anti-apoE mAbs for selective detection of apoE isoforms.
载脂蛋白 E（apoE）是脂质代谢、胆固醇转运以及脑内淀粉样 β 清除和聚集的调节剂。三种人类 apoE 同种型 apoE2、apoE3 和 apoE4 仅在一个或两个残基上有所不同。然而，其功能高度取决于同种型类型和脂化状态。在这里，我们生成了新型的抗 apoE 单克隆抗体（mAbs），并获得了一种 apoE4 选择性 mAb，其表位位于残基 110-117 内。ELISA 和生物层干涉测量表明，mAbs 的解离常数在纳摩尔范围内。使用生成的抗体，我们成功构建了夹心 ELISA 系统，该系统可以检测所有 apoE 同种型或选择性检测 apoE4。这些结果表明，所生成的抗 apoE mAbs 可用于选择性检测 apoE 同种型。
