Center for Movement Disorders, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Department of Neurology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Mov Disord. 2024 Jul;39(7):1179-1189. doi: 10.1002/mds.29798. Epub 2024 Mar 26.
Adaptive immune dysfunction may play a crucial role in Parkinson's disease (PD) development. Isolated rapid eye movement sleep behavior disorder (iRBD) represents the prodromal stage of synucleinopathies, including PD. Elucidating the peripheral adaptive immune system is crucial in iRBD, but current knowledge remains limited.
This study aimed to characterize peripheral lymphocyte profiles in iRBD patients compared with healthy control subjects (HCs).
This cross-sectional study recruited polysomnography-confirmed iRBD patients and age- and sex-matched HCs. Venous blood was collected from each participant. Flow cytometry was used to evaluate surface markers and intracellular cytokine production in peripheral blood mononuclear cells.
Forty-four iRBD patients and 36 HCs were included. Compared with HCs, patients with iRBD exhibited significant decreases in absolute counts of total lymphocytes and CD3 T cells. In terms of T cell subsets, iRBD patients showed higher frequencies and counts of proinflammatory T helper 1 cells and INF-γ CD8 T cells, along with lower frequencies and counts of anti-inflammatory T helper 2 cells. A significant increase in the frequency of central memory T cells in CD8 T cells was also observed in iRBD. Regarding B cells, iRBD patients demonstrated reduced frequencies and counts of double-negative memory B cells compared with control subjects.
This study demonstrated alterations in the peripheral adaptive immune system in iRBD, specifically in CD4 and INF-γ CD8 T cell subsets. An overall shift toward a proinflammatory state of adaptive immunity was already evident in iRBD. These observations might provide insights into the optimal timing for initiating immune interventions in PD. © 2024 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.
适应性免疫功能障碍可能在帕金森病（PD）的发展中起关键作用。孤立性快速眼动睡眠行为障碍（iRBD）代表包括 PD 在内的突触核蛋白病的前驱阶段。阐明 iRBD 中的外周适应性免疫系统至关重要，但目前的知识仍然有限。
本研究旨在比较 iRBD 患者与健康对照（HC）之间外周淋巴细胞谱。
这项横断面研究招募了经多导睡眠图（PSG）确诊的 iRBD 患者和年龄、性别匹配的 HC。从每位参与者中采集静脉血。采用流式细胞术评估外周血单个核细胞表面标志物和细胞内细胞因子的产生。
共纳入 44 例 iRBD 患者和 36 例 HC。与 HC 相比，iRBD 患者的总淋巴细胞和 CD3 T 细胞绝对值计数显著降低。在 T 细胞亚群方面，iRBD 患者表现出更高频率和计数的促炎辅助性 T 细胞 1 细胞和 INF-γ CD8 T 细胞，同时表现出更低频率和计数的抗炎辅助性 T 细胞 2 细胞。CD8 T 细胞中的中央记忆 T 细胞频率也显著增加。关于 B 细胞，iRBD 患者的双阴性记忆 B 细胞频率和计数较对照组降低。
本研究表明 iRBD 中存在外周适应性免疫系统的改变，特别是在 CD4 和 INF-γ CD8 T 细胞亚群中。适应性免疫的整体向促炎状态的转变在 iRBD 中已经很明显。这些观察结果可能为在 PD 中启动免疫干预的最佳时机提供依据。© 2024 作者。运动障碍由 Wiley 期刊出版公司代表国际帕金森病和运动障碍协会出版。
